E2 and Erns isotype-specific antibody responses in serum and oral fluid after infection with classical swine fever virus (CSFV)

Luca N. Popescu, Yaowalak Panyasing, Luis Giménez-Lirola, Jeff Zimmerman, Raymond R.R. Rowland

Research output: Contribution to journalArticlepeer-review

Abstract

Oral fluid sampling for the detection of classical swine fever virus infection provides a relatively inexpensive method for conducting active CSF surveillance. The purpose of this study was to detect CSFV nucleic acid and antibody in serum and oral fluid samples in a group of 10 pigs infected with the moderate CSFV strain, Paderborn. Based on clinical signs, outcome, and other results, pigs were placed into one of three disease outcome groups; Acute, Chronic and Recovered. Oral fluid and serum samples were analyzed for the presence of CSFV nucleic acid along with E2 and Erns surface protein-specific IgM, IgG and IgA responses. The results were summarized into a timeline of detection events beginning with the appearance of E2-IgM in serum (3 DPI) followed by CSFV nucleic acid in serum (6 DPI), CSFV nucleic acid in oral fluid (8 DPI), E2-IgG in serum (20 DPI), and E2-IgG in oral fluid (24 DPI). The results show that a combination of molecular and serological analyses of oral fluid can be incorporated into CSF surveillance.

Original languageEnglish (US)
Pages (from-to)265-269
Number of pages5
JournalVeterinary Microbiology
Volume235
DOIs
StatePublished - Aug 2019
Externally publishedYes

Keywords

  • Classical swine fever virus
  • CSFV
  • Oral fluid
  • Serology

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

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