E. Coli O157: H7 Population Reduction from Alfalfa Seeds with Malic Acid and Thiamine Dilauryl Sulfate and Quality Evaluation of the Resulting Sprouts

Lilia Fransisca, Hee Kyung Park, Hao Feng

Research output: Contribution to journalArticlepeer-review

Abstract

It has been reported that washing seeds with a 20000 ppm Ca(OCl) 2 solution as recommended by the U.S. Food and Drug Administration is unable to eliminate E. coli cells attached to seed surfaces, and the bacterial cells that have survived a sanitation wash can proliferate during sprouting to a high population. The objectives of this research were to examine the efficacy of malic acid (MA) and thiamine dilauryl sulfate (TDS) combined treatments on the inactivation of E. coli O157:H7 on alfalfa seeds, to study the growth of the remaining E. coli cells during sprouting, and to evaluate the sprout quality. When 10 g of inoculated alfalfa seeds were washed in a 10% MA-1% TDS solution, a complete elimination of E. coli was achieved. The same result was observed by washing the seeds in a 20000 ppm Ca(OCl) 2 solution. However, when the seed size was increased to 50 g while maintaining the same seed-to-sanitizer ratio, both the MA + TDS and the 20000 ppm chlorine washes failed to completely inactivate the E. coli cells on the seeds. Nevertheless, the 10% MA-1% TDS solution was significantly more effective in E. coli count reduction compared to the 20000 ppm chlorine wash. The E. coli O157:H7 cells remaining on the seeds after treatments with both sanitizers grew up to 7 to 8 log CFU/g sprout after 96 h of sprouting. Under the treatment conditions used in this study, none of the treatments resulted in significant differences in germination rate, yield, or quality of the sprouts.

Original languageEnglish (US)
Pages (from-to)M121-M126
JournalJournal of food science
Volume77
Issue number2
DOIs
StatePublished - Feb 2012

Keywords

  • Alfalfa sprout
  • E. coli
  • Malic acid
  • Surfactant
  • Thiamine dilauryl sulfate

ASJC Scopus subject areas

  • Food Science

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