Abstract
Previous data showed that complexin I, a SNARE regulatory protein, is localized in and/or around the acrosome and is necessary for the acrosome reaction in sperm. To understand how complexin I regulates the acrosome reaction, we used complexin-GST pulldown assays to identify interacting proteins. We showed that both complexins I and II bound mouse sperm dynamin 2. Dynamin 2 is a 100 kDa GTPase essential to many aspects of endocytosis but its potential role in exocytosis is unknown. Dynamin 2 is expressed in rat testis and widely expressed in other tissues; however, the function of dynamin 2 in germ cells is uncertain. Dynamin 2 protein was detected in mouse testis and was most abundant in or around the developing acrosome of spermatids. In addition, dynamin 2 was co-localized with complexin I in the acrosomal region of mammalian sperm. Its co-localization and interaction with complexin I suggest that dynamin 2 may play a role during acrosome formation and/or acrosomal exocytosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 750-757 |
| Number of pages | 8 |
| Journal | Molecular reproduction and development |
| Volume | 74 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 2007 |
Keywords
- Acrosome
- Endocytosis
- Exocytosis
- Fertilization
- SNARE
ASJC Scopus subject areas
- Genetics
- Developmental Biology
- Cell Biology