Dynamin 2 associates with complexins and is found in the acrosomal region of mammalian sperm

Longmei Zhao, Xudong Shi, Lingjun Li, David J. Miller

Research output: Contribution to journalArticlepeer-review


Previous data showed that complexin I, a SNARE regulatory protein, is localized in and/or around the acrosome and is necessary for the acrosome reaction in sperm. To understand how complexin I regulates the acrosome reaction, we used complexin-GST pulldown assays to identify interacting proteins. We showed that both complexins I and II bound mouse sperm dynamin 2. Dynamin 2 is a 100 kDa GTPase essential to many aspects of endocytosis but its potential role in exocytosis is unknown. Dynamin 2 is expressed in rat testis and widely expressed in other tissues; however, the function of dynamin 2 in germ cells is uncertain. Dynamin 2 protein was detected in mouse testis and was most abundant in or around the developing acrosome of spermatids. In addition, dynamin 2 was co-localized with complexin I in the acrosomal region of mammalian sperm. Its co-localization and interaction with complexin I suggest that dynamin 2 may play a role during acrosome formation and/or acrosomal exocytosis.

Original languageEnglish (US)
Pages (from-to)750-757
Number of pages8
JournalMolecular reproduction and development
Issue number6
StatePublished - Jun 2007


  • Acrosome
  • Endocytosis
  • Exocytosis
  • Fertilization

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology


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