TY - JOUR
T1 - Dynamical spectroscopy and microscopy of proteins in cells
AU - Gruebele, Martin
AU - Pielak, Gary J.
N1 - Funding Information:
Our research is supported by the National Science Foundation (grant number MCB-180378 to MG and MCB-1410854 to GJP) and by United States-Israel Binational Science Foundation (BSF 2017063 to GJP). We thank Jhoan Aguilar for the drawing in Figure 1 , Jens Danielsson and Mikael Oliveberg for the schematic, and Candice Crilly for help preparing the figure, Prof. Seung Joong Kim for preparing the data in Figure 3 , as well as Simon Ebbinghaus for permission to use data in Figure 2 and Elizabeth Pielak for helpful comments.
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/10
Y1 - 2021/10
N2 - With a strong understanding of how proteins fold in hand, it is now possible to ask how in-cell environments modulate their folding, binding and function. Studies accessing fast (ns to s) in-cell dynamics have accelerated over the past few years through a combination of in-cell NMR spectroscopy and time-resolved fluorescence microscopies. Here, we discuss this recent work and the emerging picture of protein surfaces as not just hydrophilic coats interfacing the solvent to the protein's core and functional regions, but as critical components in cells controlling protein mobility, function and communication with post-translational modifications.
AB - With a strong understanding of how proteins fold in hand, it is now possible to ask how in-cell environments modulate their folding, binding and function. Studies accessing fast (ns to s) in-cell dynamics have accelerated over the past few years through a combination of in-cell NMR spectroscopy and time-resolved fluorescence microscopies. Here, we discuss this recent work and the emerging picture of protein surfaces as not just hydrophilic coats interfacing the solvent to the protein's core and functional regions, but as critical components in cells controlling protein mobility, function and communication with post-translational modifications.
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U2 - 10.1016/j.sbi.2021.02.001
DO - 10.1016/j.sbi.2021.02.001
M3 - Review article
C2 - 33662744
AN - SCOPUS:85101753869
VL - 70
SP - 1
EP - 7
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
SN - 0959-440X
ER -