Dynamic measures of flagellar gene expression

Santosh Koirala, Christopher V. Rao

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Many genes are required to assemble flagella. These genes encode not only the structural elements of the flagellum but also a number of regulators that control how the flagellar genes are temporally expressed during the assembly process. These regulators also specify the likelihood that a given cell will express the flagellar genes. In particular, not all cells express the flagellar genes, resulting in mixed populations of motile and non-motile cells. Nutrients provide one signal that specifies the motile fraction. In this chapter, we describe two methods for measuring flagellar gene expression dynamics using fluorescent proteins in Salmonella enterica. Both the methods can be used to investigate the mechanisms governing flagellar gene expression dynamics.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages73-83
Number of pages11
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1593
ISSN (Print)1064-3745

Keywords

  • Flagella
  • Flow cytometry
  • Fluorescence
  • Gene expression
  • Salmonella

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Koirala, S., & Rao, C. V. (2017). Dynamic measures of flagellar gene expression. In Methods in Molecular Biology (pp. 73-83). (Methods in Molecular Biology; Vol. 1593). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6927-2_5