Dynamic label-free imaging of live-cell adhesion using photonic crystal enhanced microscopy (PCEM)

Yue Zhuo, Ji Sun Choi, Hojeong Yu, Brendan A C Harley, Brian T. Cunningham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate label-free imaging of cell attachment upon a photonic-crystal biosensor surface. Newly-implemented PCEM image-analysis software is used to dynamically visualize individual live-cell movement and demonstrate the spatiotemporal-distribution of cellular material during adhesion and motion.

Original languageEnglish (US)
Title of host publication2015 Conference on Lasers and Electro-Optics, CLEO 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781557529688
StatePublished - Aug 10 2015
EventConference on Lasers and Electro-Optics, CLEO 2015 - San Jose, United States
Duration: May 10 2015May 15 2015

Publication series

NameConference on Lasers and Electro-Optics Europe - Technical Digest
Volume2015-August

Other

OtherConference on Lasers and Electro-Optics, CLEO 2015
CountryUnited States
CitySan Jose
Period5/10/155/15/15

Fingerprint

Cell adhesion
Photonic crystals
Labels
Microscopic examination
adhesion
photonics
microscopy
Imaging techniques
cells
image analysis
bioinstrumentation
Biosensors
foams
Image analysis
crystals
attachment
Adhesion
Cells
computer programs

Keywords

  • Adhesives
  • Biosensors
  • Image color analysis
  • Imaging
  • Optical surface waves
  • Stem cells

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials

Cite this

Zhuo, Y., Choi, J. S., Yu, H., Harley, B. A. C., & Cunningham, B. T. (2015). Dynamic label-free imaging of live-cell adhesion using photonic crystal enhanced microscopy (PCEM). In 2015 Conference on Lasers and Electro-Optics, CLEO 2015 [7184049] (Conference on Lasers and Electro-Optics Europe - Technical Digest; Vol. 2015-August). Institute of Electrical and Electronics Engineers Inc..

Dynamic label-free imaging of live-cell adhesion using photonic crystal enhanced microscopy (PCEM). / Zhuo, Yue; Choi, Ji Sun; Yu, Hojeong; Harley, Brendan A C; Cunningham, Brian T.

2015 Conference on Lasers and Electro-Optics, CLEO 2015. Institute of Electrical and Electronics Engineers Inc., 2015. 7184049 (Conference on Lasers and Electro-Optics Europe - Technical Digest; Vol. 2015-August).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zhuo, Y, Choi, JS, Yu, H, Harley, BAC & Cunningham, BT 2015, Dynamic label-free imaging of live-cell adhesion using photonic crystal enhanced microscopy (PCEM). in 2015 Conference on Lasers and Electro-Optics, CLEO 2015., 7184049, Conference on Lasers and Electro-Optics Europe - Technical Digest, vol. 2015-August, Institute of Electrical and Electronics Engineers Inc., Conference on Lasers and Electro-Optics, CLEO 2015, San Jose, United States, 5/10/15.
Zhuo Y, Choi JS, Yu H, Harley BAC, Cunningham BT. Dynamic label-free imaging of live-cell adhesion using photonic crystal enhanced microscopy (PCEM). In 2015 Conference on Lasers and Electro-Optics, CLEO 2015. Institute of Electrical and Electronics Engineers Inc. 2015. 7184049. (Conference on Lasers and Electro-Optics Europe - Technical Digest).
Zhuo, Yue ; Choi, Ji Sun ; Yu, Hojeong ; Harley, Brendan A C ; Cunningham, Brian T. / Dynamic label-free imaging of live-cell adhesion using photonic crystal enhanced microscopy (PCEM). 2015 Conference on Lasers and Electro-Optics, CLEO 2015. Institute of Electrical and Electronics Engineers Inc., 2015. (Conference on Lasers and Electro-Optics Europe - Technical Digest).
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