Dynamic label-free imaging of live-cell adhesion using photonic crystal enhanced microscopy (PCEM)

Yue Zhuo, Ji Sun Choi, Hojeong Yu, Brendan A.C. Harley, Brian T. Cunningham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate label-free imaging of cell attachment upon a photonic-crystal biosensor surface. Newly-implemented PCEM image-analysis software is used to dynamically visualize individual live-cell movement and demonstrate the spatiotemporal-distribution of cellular material during adhesion and motion.

Original languageEnglish (US)
Title of host publicationCLEO
Subtitle of host publicationScience and Innovations, CLEO-SI 2015
PublisherOptical Society of America (OSA)
Number of pages1
ISBN (Electronic)9781557529688
DOIs
StatePublished - May 4 2015
EventCLEO: Science and Innovations, CLEO-SI 2015 - San Jose, United States
Duration: May 10 2015May 15 2015

Publication series

NameCLEO: Science and Innovations, CLEO-SI 2015

Other

OtherCLEO: Science and Innovations, CLEO-SI 2015
CountryUnited States
CitySan Jose
Period5/10/155/15/15

Fingerprint

Cell adhesion
Photonic crystals
Labels
Microscopic examination
adhesion
photonics
microscopy
Imaging techniques
cells
image analysis
bioinstrumentation
Biosensors
foams
Image analysis
crystals
attachment
Adhesion
Cells
computer programs

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics

Cite this

Zhuo, Y., Choi, J. S., Yu, H., Harley, B. A. C., & Cunningham, B. T. (2015). Dynamic label-free imaging of live-cell adhesion using photonic crystal enhanced microscopy (PCEM). In CLEO: Science and Innovations, CLEO-SI 2015 (CLEO: Science and Innovations, CLEO-SI 2015). Optical Society of America (OSA). https://doi.org/10.1364/CLEO_SI.2015.STh3K.7

Dynamic label-free imaging of live-cell adhesion using photonic crystal enhanced microscopy (PCEM). / Zhuo, Yue; Choi, Ji Sun; Yu, Hojeong; Harley, Brendan A.C.; Cunningham, Brian T.

CLEO: Science and Innovations, CLEO-SI 2015. Optical Society of America (OSA), 2015. (CLEO: Science and Innovations, CLEO-SI 2015).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zhuo, Y, Choi, JS, Yu, H, Harley, BAC & Cunningham, BT 2015, Dynamic label-free imaging of live-cell adhesion using photonic crystal enhanced microscopy (PCEM). in CLEO: Science and Innovations, CLEO-SI 2015. CLEO: Science and Innovations, CLEO-SI 2015, Optical Society of America (OSA), CLEO: Science and Innovations, CLEO-SI 2015, San Jose, United States, 5/10/15. https://doi.org/10.1364/CLEO_SI.2015.STh3K.7
Zhuo Y, Choi JS, Yu H, Harley BAC, Cunningham BT. Dynamic label-free imaging of live-cell adhesion using photonic crystal enhanced microscopy (PCEM). In CLEO: Science and Innovations, CLEO-SI 2015. Optical Society of America (OSA). 2015. (CLEO: Science and Innovations, CLEO-SI 2015). https://doi.org/10.1364/CLEO_SI.2015.STh3K.7
Zhuo, Yue ; Choi, Ji Sun ; Yu, Hojeong ; Harley, Brendan A.C. ; Cunningham, Brian T. / Dynamic label-free imaging of live-cell adhesion using photonic crystal enhanced microscopy (PCEM). CLEO: Science and Innovations, CLEO-SI 2015. Optical Society of America (OSA), 2015. (CLEO: Science and Innovations, CLEO-SI 2015).
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