TY - JOUR
T1 - Dynamic Imaging Reveals Coordinate Effects of Cyclic Stretch and Substrate Stiffness on Endothelial Integrity
AU - Dan, Arkaprava
AU - Huang, Ryan B.
AU - Leckband, Deborah E.
N1 - Publisher Copyright:
© 2016, Biomedical Engineering Society.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - We describe an equibiaxial cell stretcher and hybrid, elastic membrane platform designed for dynamic imaging of cells on substrates with physiological stiffness undergoing cyclic stretch. Studies enabled by this device revealed that both substrate stiffness and cyclic stretch coordinately protect pulmonary endothelial monolayers against thrombin-induced disruption. The fluorescence imaging possible with the designed hybrid membranes further revealed similarities and differences in actin and cell dynamics during monolayer recovery. The improved live-cell imaging capabilities of this platform, when used in conjunction with fluorescent probes, will have broad applications for investigations of the impact of biochemical stimuli and mechanotransduction mechanisms on mechanically perturbed tissues.
AB - We describe an equibiaxial cell stretcher and hybrid, elastic membrane platform designed for dynamic imaging of cells on substrates with physiological stiffness undergoing cyclic stretch. Studies enabled by this device revealed that both substrate stiffness and cyclic stretch coordinately protect pulmonary endothelial monolayers against thrombin-induced disruption. The fluorescence imaging possible with the designed hybrid membranes further revealed similarities and differences in actin and cell dynamics during monolayer recovery. The improved live-cell imaging capabilities of this platform, when used in conjunction with fluorescent probes, will have broad applications for investigations of the impact of biochemical stimuli and mechanotransduction mechanisms on mechanically perturbed tissues.
KW - Cell stretcher
KW - Dynamic fluorescence imaging
KW - Endothelial mechanics
KW - Mechanotransduction
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UR - http://www.scopus.com/inward/citedby.url?scp=84975110877&partnerID=8YFLogxK
U2 - 10.1007/s10439-016-1677-4
DO - 10.1007/s10439-016-1677-4
M3 - Article
C2 - 27317301
AN - SCOPUS:84975110877
SN - 0090-6964
VL - 44
SP - 3655
EP - 3667
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
IS - 12
ER -