@article{9bbc66cd23de4639a54778939e8fde75,
title = "During photosynthetic induction, biochemical and stomatal limitations differ between Brassica crops",
abstract = "Interventions to increase crop radiation use efficiency rely on understanding of how biochemical and stomatal limitations affect photosynthesis. When leaves transition from shade to high light, slow increases in maximum Rubisco carboxylation rate and stomatal conductance limit net CO2 assimilation for several minutes. However, as stomata open intercellular [CO2] increases, so electron transport rate could also become limiting. Photosynthetic limitations were evaluated in three important Brassica crops: Brassica rapa, Brassica oleracea and Brassica napus. Measurements of induction after a period of shade showed that net CO2 assimilation by B. rapa and B. napus saturated by 10 min. A new method of analyzing limitations to induction by varying intercellular [CO2] showed this was due to co-limitation by Rubisco and electron transport. By contrast, in B. oleracea persistent Rubisco limitation meant that CO2 assimilation was still recovering 15 min after induction. Correspondingly, B. oleracea had the lowest Rubisco total activity. The methodology developed, and its application here, shows a means to identify the basis of variation in photosynthetic efficiency in fluctuating light, which could be exploited in breeding and bioengineering to improve crop productivity.",
keywords = "Brassica napus, Brassica oleracea, Brassica rapa, CO response, Rubisco, crop improvement, dynamic photosynthesis, photosynthetic electron transport, photosynthetic induction, stomata",
author = "Taylor, {Samuel H.} and Orr, {Douglas J.} and Elizabete Carmo-Silva and Long, {Stephen P.}",
note = "Funding Information: This work was supported by Lancaster University, and by a subaward from the University of Illinois as part of the research project Realising Increased Photosynthetic Efficiency (RIPE) that is funded by the Bill & Melinda Gates Foundation, Foundation for Food and Agriculture Research, and the U.K. Foreign, Commonwealth and Development Office under grant number OPP1172157. The authors thank George Goodwin (Elsoms Seeds Ltd.) and Graham Teakle (Warwick Crop Centre) for providing seeds; Dr. Shaun Nielsen for discussions around R programming for model fitting; and two anonymous reviewers and Prof. A.P.M. Weber for constructive feedback that improved the manuscript. Funding Information: This work was supported by Lancaster University, and by a subaward from the University of Illinois as part of the research project Realising Increased Photosynthetic Efficiency (RIPE) that is funded by the Bill & Melinda Gates Foundation, Foundation for Food and Agriculture Research, and the U.K. Foreign, Commonwealth and Development Office under grant number OPP1172157. The authors thank George Goodwin (Elsoms Seeds Ltd.) and Graham Teakle (Warwick Crop Centre) for providing seeds; Dr. Shaun Nielsen for discussions around R programming for model fitting; and two anonymous reviewers and Prof. A.P.M. Weber for constructive feedback that improved the manuscript. Publisher Copyright: {\textcopyright} 2020 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.",
year = "2020",
month = nov,
day = "1",
doi = "10.1111/pce.13862",
language = "English (US)",
volume = "43",
pages = "2623--2636",
journal = "Plant, Cell and Environment",
issn = "0140-7791",
publisher = "Wiley-Blackwell",
number = "11",
}