TY - JOUR
T1 - Duodenal infusion of α-linolenic acid affects fatty acid metabolism in the mammary gland of lactating dairy cows
AU - Yang, G.
AU - Bu, D. P.
AU - Wang, J. Q.
AU - Khas-Erdene,
AU - Zhou, L. Y.
AU - Loor, J. J.
N1 - Funding Information:
This work was supported by the National Key Basic Research Program of China (Beijing, China; no. 2011CB100805). The authors thank Richard Kellems (Plant and Animal Sciences Department, Brigham Young University, Provo, UT) for helpful suggestions during the writing of the manuscript.
PY - 2012/10
Y1 - 2012/10
N2 - Increasing the concentration of α-linolenic acid (LNA; 18:3 cis-9,. cis-12,. cis-15) in blood might affect fatty acid (FA) metabolism in the mammary gland of dairy cows. The objective was to determine the effects of different arterial concentrations of 18:3 cis-9,. cis-12,. cis-15 (18:3n-3) achieved via duodenal infusions with LNA on mammary uptake [assessed via arterial-rectificative venous concentration (AC-RVC) differences], synthesis of FA, and mammary gland FA balance in lactating dairy cows. Four primiparous lactating Chinese Holstein cows fitted with duodenal cannulas were administered 2 treatments in a crossover design: LNA-rich FA infusion at varying concentrations (0, 100, 200, and 300 g/d) versus basal infusate control. Arterial concentration of 18:3n-3 increased quadratically (29.24, 134.1, 218.3, and 219.3. mg/L of plasma) as LNA infusion levels increased from 0 to 300 g/d. The mammary extraction rate and uptake of 18:3n-3 increased linearly as LNA infusion increased. The AC-RVC difference of total FA and 18:3n-3 increased more rapidly than arterial concentrations with all treatments. Increasing LNA infusion increased linearly the balance of 10:0 and 12:0, whereas it decreased linearly the 14:1 and 15:0 balances. Increasing arterial concentration of 18:3n-3 affects uptake and synthesis of FA in the mammary gland of lactating dairy cows. It is also suggested that the use of AC-RVC difference maybe an acceptable way to investigate mammary gland uptake and synthesis of FA.
AB - Increasing the concentration of α-linolenic acid (LNA; 18:3 cis-9,. cis-12,. cis-15) in blood might affect fatty acid (FA) metabolism in the mammary gland of dairy cows. The objective was to determine the effects of different arterial concentrations of 18:3 cis-9,. cis-12,. cis-15 (18:3n-3) achieved via duodenal infusions with LNA on mammary uptake [assessed via arterial-rectificative venous concentration (AC-RVC) differences], synthesis of FA, and mammary gland FA balance in lactating dairy cows. Four primiparous lactating Chinese Holstein cows fitted with duodenal cannulas were administered 2 treatments in a crossover design: LNA-rich FA infusion at varying concentrations (0, 100, 200, and 300 g/d) versus basal infusate control. Arterial concentration of 18:3n-3 increased quadratically (29.24, 134.1, 218.3, and 219.3. mg/L of plasma) as LNA infusion levels increased from 0 to 300 g/d. The mammary extraction rate and uptake of 18:3n-3 increased linearly as LNA infusion increased. The AC-RVC difference of total FA and 18:3n-3 increased more rapidly than arterial concentrations with all treatments. Increasing LNA infusion increased linearly the balance of 10:0 and 12:0, whereas it decreased linearly the 14:1 and 15:0 balances. Increasing arterial concentration of 18:3n-3 affects uptake and synthesis of FA in the mammary gland of lactating dairy cows. It is also suggested that the use of AC-RVC difference maybe an acceptable way to investigate mammary gland uptake and synthesis of FA.
KW - Dairy cow
KW - Mammary gland metabolism
KW - Uptake
KW - α-linolenic acid
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U2 - 10.3168/jds.2011-4893
DO - 10.3168/jds.2011-4893
M3 - Article
C2 - 22921622
AN - SCOPUS:84867100496
SN - 0022-0302
VL - 95
SP - 5821
EP - 5830
JO - Journal of Dairy Science
JF - Journal of Dairy Science
IS - 10
ER -