DsbL and DsbI contribute to periplasmic disulfide bond formation in Salmonella enterica serovar Typhimurium

Dongxia Lin, Byoungkwan Kim, James M. Slauch

Research output: Contribution to journalArticle

Abstract

Disulfide bond formation in periplasmic proteins is catalysed by the DsbA/DsbB system in most Gram-negative bacteria. Salmonella enterica serovar Typhimurium also encodes a paralogous pair of proteins to DsbA and DsbB, DsbL and DsbI, respectively, downstream of a periplasmic arylsulfate sulfotransferase (ASST). We show that DsbL and DsbI function as a redox pair contributing to periplasmic disulfide bond formation and, as such, affect transcription of the Salmonella pathogenicity island 1 (SPI1) type three secretion system genes and activation of the RcsCDB system, as well as ASST activity. In contrast to DsbA/DsbB, however, the DsbL/DsbI system cannot catalyse the disulfide bond formation required for flagellar assembly. Phylogenic analysis suggests that the assT dsbL dsbI genes are ancestral in the Enterobacteriaceae, but have been lost in many lineages. Deletion of assT confers no virulence defect during acute Salmonella infection of mice.

Original languageEnglish (US)
Pages (from-to)4014-4024
Number of pages11
JournalMicrobiology
Volume155
Issue number12
DOIs
StatePublished - Dec 11 2009

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arylsulfate sulfotransferase
Salmonella enterica
Disulfides
Periplasmic Proteins
Genomic Islands
Salmonella Infections
Enterobacteriaceae
Gram-Negative Bacteria
Salmonella
Transcriptional Activation
Oxidation-Reduction
Virulence
Genes
Serogroup
Proteins

ASJC Scopus subject areas

  • Microbiology

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DsbL and DsbI contribute to periplasmic disulfide bond formation in Salmonella enterica serovar Typhimurium. / Lin, Dongxia; Kim, Byoungkwan; Slauch, James M.

In: Microbiology, Vol. 155, No. 12, 11.12.2009, p. 4014-4024.

Research output: Contribution to journalArticle

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AB - Disulfide bond formation in periplasmic proteins is catalysed by the DsbA/DsbB system in most Gram-negative bacteria. Salmonella enterica serovar Typhimurium also encodes a paralogous pair of proteins to DsbA and DsbB, DsbL and DsbI, respectively, downstream of a periplasmic arylsulfate sulfotransferase (ASST). We show that DsbL and DsbI function as a redox pair contributing to periplasmic disulfide bond formation and, as such, affect transcription of the Salmonella pathogenicity island 1 (SPI1) type three secretion system genes and activation of the RcsCDB system, as well as ASST activity. In contrast to DsbA/DsbB, however, the DsbL/DsbI system cannot catalyse the disulfide bond formation required for flagellar assembly. Phylogenic analysis suggests that the assT dsbL dsbI genes are ancestral in the Enterobacteriaceae, but have been lost in many lineages. Deletion of assT confers no virulence defect during acute Salmonella infection of mice.

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