Drug metabolite-specific lymphocyte responses in sulfamethoxazole allergic patients with cystic fibrosis

Sidonie N. Lavergne, Paul Whitaker, Daniel Peckham, Steve Conway, B. Kevin Park, Dean J. Naisbitt

Research output: Contribution to journalArticlepeer-review

Abstract

Sulfamethoxazole (SMX) is an important antibiotic in the management of patients with cystic fibrosis, but allergic reactions may develop thus restricting therapy. The aim of this study was to utilize drug (metabolite) antigens to diagnose SMX-mediated allergic reactions in patients with cystic fibrosis. Lymphocytes from 2/12 allergic patients were stimulated to proliferate strongly with the SMX metabolite nitroso SMX (SMX-NO). In contrast, responses to SMX were weak. The introduction of an antigen-driven T-cell enrichment step prior to the analysis of proliferation increased the sensitivity of the assay. SMX-NO responses were detected with lymphocytes from all patients with cutaneous signs.

Original languageEnglish (US)
Pages (from-to)1009-1011
Number of pages3
JournalChemical Research in Toxicology
Volume23
Issue number6
DOIs
StatePublished - Jun 21 2010
Externally publishedYes

ASJC Scopus subject areas

  • Toxicology

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