Double-strand end repair via the RecBC pathway in Escherichia coli primes DNA replication

Andrei Kuzminov, Franklin W. Stahl

Research output: Contribution to journalArticlepeer-review


To study the relationship between homologous recombination and DNA replication in Escherichia coli, we monitored the behavior of phage λ chromosomes, repressed or not for λ gene activities. Recombination in our system is stimulated both by DNA replication and by experimentally introduced double-strand ends, supporting the idea that DNA replication generates occasional double-strand ends. We report that the RecBC recombinational pathway of E. coli uses double-strand ends to prime DNA synthesis, implying a circular relationship between DNA replication and recombination and suggesting that the primary role of recombination is in the repair of disintegrated replication forks arising during vegetative reproduction.

Original languageEnglish (US)
Pages (from-to)345-356
Number of pages12
JournalGenes and Development
Issue number3
StatePublished - Feb 1 1999
Externally publishedYes


  • DNA replication
  • DSB repair
  • Homologous recombination
  • RecA
  • RecBC
  • RecD

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology


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