TY - JOUR
T1 - Donor Sex and Passage Conditions Influence MSC Osteogenic Response in Mineralized Collagen Scaffolds
AU - Kolliopoulos, Vasiliki
AU - Tiffany, Aleczandria
AU - Polanek, Maxwell
AU - Harley, Brendan A. C.
N1 - The authors would like to acknowledge the following institutes for access to their facilities and services: the School of Chemical Sciences Microanalysis Laboratory, the Carl R. Woese Institute for Genomic Biology, the Tumor Engineering and Phenotyping Shared Resource (TEP) at the Cancer Center at Illinois, and the Beckman Institute for Advanced Science and Technology, located at the University of Illinois. Research reported in this publication was supported by the National Institute of Dental and Craniofacial Research of the National Institutes of Health under Award Number R21 DE026582 and R01 DE030491 (BACH) as well as the National Institute of Arthritis and Musculoskeletal and Skin Diseases under Award Number R01 AR077858 (BACH). The authors are also grateful for funds provided by the NSF Graduate Research Fellowship (DGE-1746047 to VK; DGE-1144245 to A.S.T.) and the Chemistry-Biology Interface Research Training Program at the University of Illinois (T32 GM070421, V.K.). Additional support was provided by the Carl R. Woese Institute for Genomic Biology and the Chemical and Biomolecular Engineering Dept. at the University of Illinois at Urbana-Champaign. The interpretations and conclusions presented are those of the authors and are not necessarily endorsed by the National Institutes of Health or the National Science Foundation.
The authors would like to acknowledge the following institutes for access to their facilities and services: the School of Chemical Sciences Microanalysis Laboratory, the Carl R. Woese Institute for Genomic Biology, the Tumor Engineering and Phenotyping Shared Resource (TEP) at the Cancer Center at Illinois, and the Beckman Institute for Advanced Science and Technology, located at the University of Illinois. Research reported in this publication was supported by the National Institute of Dental and Craniofacial Research of the National Institutes of Health under Award Number R21 DE026582 and R01 DE030491 (BACH) as well as the National Institute of Arthritis and Musculoskeletal and Skin Diseases under Award Number R01 AR077858 (BACH). The authors are also grateful for funds provided by the NSF Graduate Research Fellowship (DGE\u20101746047 to VK; DGE\u20101144245 to A.S.T.) and the Chemistry\u2010Biology Interface Research Training Program at the University of Illinois (T32 GM070421, V.K.). Additional support was provided by the Carl R. Woese Institute for Genomic Biology and the Chemical and Biomolecular Engineering Dept. at the University of Illinois at Urbana\u2010Champaign. The interpretations and conclusions presented are those of the authors and are not necessarily endorsed by the National Institutes of Health or the National Science Foundation.
PY - 2024/10/28
Y1 - 2024/10/28
N2 - Contemporary tissue engineering efforts often seek to use mesenchymal stem cells (MSCs) due to their multi-potent potential and ability to generate a pro-regenerative secretome. While many have reported the influence of matrix environment on MSC osteogenic response, few have investigated the effects of donor and sex. Here, a well-defined mineralized collagen scaffold is used to study the influence of passage number and donor-reported sex on MSC proliferation and osteogenic potential. A library of bone marrow and adipose tissue-derived stem cells from eight donors to examine donor viability in osteogenic capacity in mineralized collagen scaffolds is obtained. MSCs displayed reduced proliferative capacity as a function of passage duration. Further, MSCs showed significant sex-associated variability in osteogenic capacity. Notably, MSCs from male donors displayed significantly higher cell proliferation while MSCs from female donors displayed significantly higher osteogenic response via increased alkaline phosphate activity, osteoprotegerin release, and mineral formation in vitro. The study highlights the essentiality of including donor-reported sex as an experimental variable and reporting culture expansion in future studies of biomaterial regenerative potential.
AB - Contemporary tissue engineering efforts often seek to use mesenchymal stem cells (MSCs) due to their multi-potent potential and ability to generate a pro-regenerative secretome. While many have reported the influence of matrix environment on MSC osteogenic response, few have investigated the effects of donor and sex. Here, a well-defined mineralized collagen scaffold is used to study the influence of passage number and donor-reported sex on MSC proliferation and osteogenic potential. A library of bone marrow and adipose tissue-derived stem cells from eight donors to examine donor viability in osteogenic capacity in mineralized collagen scaffolds is obtained. MSCs displayed reduced proliferative capacity as a function of passage duration. Further, MSCs showed significant sex-associated variability in osteogenic capacity. Notably, MSCs from male donors displayed significantly higher cell proliferation while MSCs from female donors displayed significantly higher osteogenic response via increased alkaline phosphate activity, osteoprotegerin release, and mineral formation in vitro. The study highlights the essentiality of including donor-reported sex as an experimental variable and reporting culture expansion in future studies of biomaterial regenerative potential.
KW - bone tissue engineering
KW - donor variability
KW - mesenchymal stem cells
KW - sex variation
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U2 - 10.1002/adhm.202400039
DO - 10.1002/adhm.202400039
M3 - Article
C2 - 39036820
SN - 2192-2640
VL - 13
JO - Advanced Healthcare Materials
JF - Advanced Healthcare Materials
IS - 27
M1 - 2400039
ER -