Domains of heterochromatin protein 1 required for Drosophila melanogaster heterochromatin spreading

Karrie A. Hines, Diane E. Cryderman, Kaitlin M. Flannery, Yang Hongbo, Michael W. Vitalini, Tulle Hazelrigg, Craig A. Mizzen, Lori L. Wallrath

Research output: Contribution to journalArticle

Abstract

Centric regions of eukaryotic genomes are packaged into heterochromatin, which possesses the ability to spread along the chromosome and silence gene expression. The process of spreading has been challenging to study at the molecular level due to repetitious sequences within centric regions. A heterochromatin protein 1 (HP1) tethering system was developed that generates "ectopic heterochromatin" at sites within euchromatic regions of the Drosophila melanogaster genome. Using this system, we show that HP1 dimerization and the PxVxL interaction platform formed by dimerization of the HP1 chromo shadow domain are necessary for spreading to a downstream reporter gene located 3.7 kb away. Surprisingly, either the HP1 chromo domain or the chromo shadow domain alone is sufficient for spreading and silencing at a downstream reporter gene located 1.9 kb away. Spreading is dependent on at least two H3K9 methyltransferases, with SU(VAR)3-9 playing a greater role at the 3.7-kb reporter and dSETDB1 predominately acting at the 1.9 kb reporter. These data support a model whereby HP1 takes part in multiple mechanisms of silencing and spreading.

Original languageEnglish (US)
Pages (from-to)967-977
Number of pages11
JournalGenetics
Volume182
Issue number4
DOIs
StatePublished - Aug 1 2009

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Heterochromatin
Drosophila melanogaster
Reporter Genes
Protein Multimerization
Genome
Methyltransferases
Dimerization
Chromosomes
heterochromatin-specific nonhistone chromosomal protein HP-1
Gene Expression

ASJC Scopus subject areas

  • Genetics

Cite this

Hines, K. A., Cryderman, D. E., Flannery, K. M., Hongbo, Y., Vitalini, M. W., Hazelrigg, T., ... Wallrath, L. L. (2009). Domains of heterochromatin protein 1 required for Drosophila melanogaster heterochromatin spreading. Genetics, 182(4), 967-977. https://doi.org/10.1534/genetics.109.105338

Domains of heterochromatin protein 1 required for Drosophila melanogaster heterochromatin spreading. / Hines, Karrie A.; Cryderman, Diane E.; Flannery, Kaitlin M.; Hongbo, Yang; Vitalini, Michael W.; Hazelrigg, Tulle; Mizzen, Craig A.; Wallrath, Lori L.

In: Genetics, Vol. 182, No. 4, 01.08.2009, p. 967-977.

Research output: Contribution to journalArticle

Hines, KA, Cryderman, DE, Flannery, KM, Hongbo, Y, Vitalini, MW, Hazelrigg, T, Mizzen, CA & Wallrath, LL 2009, 'Domains of heterochromatin protein 1 required for Drosophila melanogaster heterochromatin spreading', Genetics, vol. 182, no. 4, pp. 967-977. https://doi.org/10.1534/genetics.109.105338
Hines KA, Cryderman DE, Flannery KM, Hongbo Y, Vitalini MW, Hazelrigg T et al. Domains of heterochromatin protein 1 required for Drosophila melanogaster heterochromatin spreading. Genetics. 2009 Aug 1;182(4):967-977. https://doi.org/10.1534/genetics.109.105338
Hines, Karrie A. ; Cryderman, Diane E. ; Flannery, Kaitlin M. ; Hongbo, Yang ; Vitalini, Michael W. ; Hazelrigg, Tulle ; Mizzen, Craig A. ; Wallrath, Lori L. / Domains of heterochromatin protein 1 required for Drosophila melanogaster heterochromatin spreading. In: Genetics. 2009 ; Vol. 182, No. 4. pp. 967-977.
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