TY - JOUR
T1 - Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice
AU - Roqueta-Rivera, Manuel
AU - Stroud, Chad K.
AU - Haschek, Wanda M.
AU - Akare, Sandeep J.
AU - Segre, Mariangela
AU - Brush, Richard S.
AU - Agbaga, Martin Paul
AU - Anderson, Robert E.
AU - Hess, Rex A.
AU - Nakamura, Manabu T.
PY - 2010/2/1
Y1 - 2010/2/1
N2 - Delta-6 desaturase-null mice ( / ) are unable to synthesize highly unsaturated fatty acids (HUFAs): arachidonic acid (AA), docosahexaenoic acid (DHA), and n6-docosapentaenoic acid (DPAn6). The / males exhibit infertility and arrest of spermatogenesis at late spermiogenesis. To determine which HUFA is essential for spermiogenesis, a diet supplemented with either 0.2% (w/w) AA or DHA was fed to wild-type (+/+) and / males at weaning until 16 weeks of age (n = 3-5). A breeding success rate of DHA-supplemented - /was comparable to +/+. DHA-fed/ - showed normal sperm counts and spermiogenesis. Dietary AA was less effective in restoring fertility, sperm count, and spermiogenesis than DHA. Testis fatty acid analysis showed restored DHA in DHA-fed / , but DPAn6 remained depleted. In AA-fed / AA was restored at the +/+ level, and 22:4n6, an AA elongated product, accumulated in testis. Cholesta-3,5-diene was present in testis of +/+ and DHAfed / , whereas it diminished in / and AA-fed / , suggesting impaired sterol metabolism in these groups. Expression of spermiogenesis marker genes was largely normal in all groups. In conclusion, DHA was capable of restoring all observed impairment in male reproduction, whereas 22:4n6 formed from dietary AA may act as an inferior substitute for DHA.-Roqueta-Rivera, M., C. K. Stroud, W. M. Haschek, S. J. Akare, M. Segre, R. S. Brush, M-P. Agbaga, R. E. Anderson, R. A. Hess, and M. T. Nakamura. Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice.
AB - Delta-6 desaturase-null mice ( / ) are unable to synthesize highly unsaturated fatty acids (HUFAs): arachidonic acid (AA), docosahexaenoic acid (DHA), and n6-docosapentaenoic acid (DPAn6). The / males exhibit infertility and arrest of spermatogenesis at late spermiogenesis. To determine which HUFA is essential for spermiogenesis, a diet supplemented with either 0.2% (w/w) AA or DHA was fed to wild-type (+/+) and / males at weaning until 16 weeks of age (n = 3-5). A breeding success rate of DHA-supplemented - /was comparable to +/+. DHA-fed/ - showed normal sperm counts and spermiogenesis. Dietary AA was less effective in restoring fertility, sperm count, and spermiogenesis than DHA. Testis fatty acid analysis showed restored DHA in DHA-fed / , but DPAn6 remained depleted. In AA-fed / AA was restored at the +/+ level, and 22:4n6, an AA elongated product, accumulated in testis. Cholesta-3,5-diene was present in testis of +/+ and DHAfed / , whereas it diminished in / and AA-fed / , suggesting impaired sterol metabolism in these groups. Expression of spermiogenesis marker genes was largely normal in all groups. In conclusion, DHA was capable of restoring all observed impairment in male reproduction, whereas 22:4n6 formed from dietary AA may act as an inferior substitute for DHA.-Roqueta-Rivera, M., C. K. Stroud, W. M. Haschek, S. J. Akare, M. Segre, R. S. Brush, M-P. Agbaga, R. E. Anderson, R. A. Hess, and M. T. Nakamura. Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice.
KW - Arachidonic acid
KW - Choelsta-3,5-diene
KW - Essential fatty acids
KW - Highly unsaturated fatty acids
KW - Male reproduction
KW - Spermiogenesis
KW - Very-long-chainpolyunsaturated fatty acids
UR - http://www.scopus.com/inward/record.url?scp=77949535934&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77949535934&partnerID=8YFLogxK
U2 - 10.1194/jlr.M001180
DO - 10.1194/jlr.M001180
M3 - Article
C2 - 19690334
AN - SCOPUS:77949535934
SN - 0022-2275
VL - 51
SP - 360
EP - 367
JO - Journal of Lipid Research
JF - Journal of Lipid Research
IS - 2
ER -