DNase-capture reveals differential transcription factor binding modalities

Daniel Kang, Richard Sherwood, Amira Barkal, Tatsunori Hashimoto, Logan Engstrom, David Gifford

Research output: Contribution to journalArticlepeer-review

Abstract

We describe DNase-capture, an assay that increases the analytical resolution of DNase-seq by focusing its sequencing phase on selected genomic regions. We introduce a new method to compensate for capture bias called BaseNormal that allows for accurate recovery of transcription factor protection profiles from DNase-capture data. We show that these normalized data allow for nuanced detection of transcription factor binding heterogeneity with as few as dozens of sites.

Original languageEnglish (US)
Article numbere0187046
JournalPloS one
Volume12
Issue number12
DOIs
StatePublished - Dec 2017
Externally publishedYes

ASJC Scopus subject areas

  • General

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