TY - JOUR
T1 - DNA methylation profiles differ between field- and in vitro-grown leaves of apple
AU - Li, Xiangqian
AU - Xu, Mingliang
AU - Korban, Schuyler S.
N1 - Funding Information:
Acknowledgements. This research was funded by a grant from the Illinois Council for Food and Agriculture Research-External program.
PY - 2002/11/1
Y1 - 2002/11/1
N2 - A reliable method has been previously developed to detect cytosine methylation at the 5′-CCGG-3′ sequence using isoschizomers (MspI and HpaII) and a modified amplified fragment length polymorphism (AFLP) technique. With this method, DNA methylation profiles were investigated in leaf tissues of apple (Malus x domestica cv. Gala) grown under two different growth conditions, field and tissue culture A. total of 1,622 AFLP bands were detected using 32 pairs of primers, and these banding patterns were assembled into three groups. Type I AFLP bands were present in both EcoR I/Hpa II and EcoR I/Msp I lanes. Type II bands were present in the EcoR I/Msp I lanes, but not in EcoR I/Hpa II lanes. Type III bands were present in EcoR I/Hpa II lanes, but not in the EcoR I/Msp I lanes. For leaf tissues of field- and in vitro-grown apples, the ratios of types I, II, and III to the total number of amplified fragments were 70%, 24%, and 6%, and 71%, 23%, and 6%, respectively. Although the ratios of the three types of banding patterns were similar in both leaf tissues, a few bands specific to either field-grown trees or in vitro-grown shoots were observed This study provided evidence that changes in methylation occurred in apple leaf tissues subjected to tissue culture growth conditions.
AB - A reliable method has been previously developed to detect cytosine methylation at the 5′-CCGG-3′ sequence using isoschizomers (MspI and HpaII) and a modified amplified fragment length polymorphism (AFLP) technique. With this method, DNA methylation profiles were investigated in leaf tissues of apple (Malus x domestica cv. Gala) grown under two different growth conditions, field and tissue culture A. total of 1,622 AFLP bands were detected using 32 pairs of primers, and these banding patterns were assembled into three groups. Type I AFLP bands were present in both EcoR I/Hpa II and EcoR I/Msp I lanes. Type II bands were present in the EcoR I/Msp I lanes, but not in EcoR I/Hpa II lanes. Type III bands were present in EcoR I/Hpa II lanes, but not in the EcoR I/Msp I lanes. For leaf tissues of field- and in vitro-grown apples, the ratios of types I, II, and III to the total number of amplified fragments were 70%, 24%, and 6%, and 71%, 23%, and 6%, respectively. Although the ratios of the three types of banding patterns were similar in both leaf tissues, a few bands specific to either field-grown trees or in vitro-grown shoots were observed This study provided evidence that changes in methylation occurred in apple leaf tissues subjected to tissue culture growth conditions.
KW - AFLP
KW - Apple
KW - Cytosine methylation
KW - Isoschizomers
KW - Malus
KW - Tissue culture
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U2 - 10.1078/0176-1617-00899
DO - 10.1078/0176-1617-00899
M3 - Article
AN - SCOPUS:0036858619
SN - 0176-1617
VL - 159
SP - 1229
EP - 1234
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
IS - 11
ER -