DNA ejection from bacteriophage: Towards a general behavior for osmotic-suppression experiments

M. Castelnovo, A. Evilevitch

Research output: Contribution to journalArticlepeer-review

Abstract

We present in this work in vitro measurements of the force ejecting DNA from two distinct bacteriophages (T5 and λ using the osmotic-suppression technique. Our data are analyzed by revisiting the current theories of DNA packaging in spherical capsids. In particular we show that a simplified analytical model based on bending considerations only is able to account quantitatively for the experimental findings. Physical and biological consequences are discussed.

Original languageEnglish (US)
Pages (from-to)9-18
Number of pages10
JournalEuropean Physical Journal E
Volume24
Issue number1
DOIs
StatePublished - Sep 2007
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • General Chemistry
  • General Materials Science
  • Surfaces and Interfaces

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