DNA content analysis of smooth pigweed (Amaranthus hybridus) and tall waterhemp (A. tuberculatus): Implications for hybrid detection

Mark R. Jeschke; Patrick J. Tranel; A. Lane Rayburn

doi:10.1614/0043-1745(2003)051[0001:DCAOSP]2.0.CO;2

DNA content analysis of smooth pigweed (Amaranthus hybridus) and tall waterhemp (A. tuberculatus): Implications for hybrid detection

Mark R. Jeschke, Patrick J. Tranel, A. Lane Rayburn

Crop Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Prior research indicated that analysis of nuclear DNA content by flow cytometry could be used to distinguish smooth pigweed × tall waterhemp hybrids when the parent lines are known. Flow cytometry was performed on nuclei isolated from several Illinois populations of smooth pigweed and tall waterhemp. The smooth pigweed and tall waterhemp analyzed had nonoverlapping 2C nuclear DNA content values, with mean values of 1.04 and 1.34 pg, respectively. The consistent difference in DNA content observed between the two species indicates that DNA content analysis can be used to distinguish their hybrid progeny in natural populations.

Original language	English (US)
Pages (from-to)	1-3
Number of pages	3
Journal	Weed Science
Volume	51
Issue number	1
DOIs	https://doi.org/10.1614/0043-1745(2003)051[0001:DCAOSP]2.0.CO;2
State	Published - 2003

Keywords

DNA content
Flow cytometry
Hybridization
Intraspecific variation

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

Online availability

10.1614/0043-1745(2003)051[0001:DCAOSP]2.0.CO;2

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T1 - DNA content analysis of smooth pigweed (Amaranthus hybridus) and tall waterhemp (A. tuberculatus)

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AU - Jeschke, Mark R.

AU - Tranel, Patrick J.

AU - Rayburn, A. Lane

PY - 2003

Y1 - 2003

N2 - Prior research indicated that analysis of nuclear DNA content by flow cytometry could be used to distinguish smooth pigweed × tall waterhemp hybrids when the parent lines are known. Flow cytometry was performed on nuclei isolated from several Illinois populations of smooth pigweed and tall waterhemp. The smooth pigweed and tall waterhemp analyzed had nonoverlapping 2C nuclear DNA content values, with mean values of 1.04 and 1.34 pg, respectively. The consistent difference in DNA content observed between the two species indicates that DNA content analysis can be used to distinguish their hybrid progeny in natural populations.

AB - Prior research indicated that analysis of nuclear DNA content by flow cytometry could be used to distinguish smooth pigweed × tall waterhemp hybrids when the parent lines are known. Flow cytometry was performed on nuclei isolated from several Illinois populations of smooth pigweed and tall waterhemp. The smooth pigweed and tall waterhemp analyzed had nonoverlapping 2C nuclear DNA content values, with mean values of 1.04 and 1.34 pg, respectively. The consistent difference in DNA content observed between the two species indicates that DNA content analysis can be used to distinguish their hybrid progeny in natural populations.

KW - DNA content

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KW - Hybridization

KW - Intraspecific variation

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DNA content analysis of smooth pigweed (Amaranthus hybridus) and tall waterhemp (A. tuberculatus): Implications for hybrid detection

Abstract

Keywords

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