DNA-catalyzed sequence-specific hydrolysis of DNA

Madhavaiah Chandra, Amit Sachdeva, Scott K Silverman

Research output: Contribution to journalArticle

Abstract

Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and with a rate enhancement of 108 (possibly as high as 1014). The new DNA catalysts require both Mn2+ and Zn2+, which is noteworthy because many natural DNA nucleases are bimetallic protein enzymes.

Original languageEnglish (US)
Pages (from-to)718-720
Number of pages3
JournalNature chemical biology
Volume5
Issue number10
DOIs
StatePublished - Oct 2009

Fingerprint

Hydrolysis
Catalytic DNA
DNA
RNA Cleavage
Deoxyribonucleases
Peptides
Enzymes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

DNA-catalyzed sequence-specific hydrolysis of DNA. / Chandra, Madhavaiah; Sachdeva, Amit; Silverman, Scott K.

In: Nature chemical biology, Vol. 5, No. 10, 10.2009, p. 718-720.

Research output: Contribution to journalArticle

Chandra, Madhavaiah ; Sachdeva, Amit ; Silverman, Scott K. / DNA-catalyzed sequence-specific hydrolysis of DNA. In: Nature chemical biology. 2009 ; Vol. 5, No. 10. pp. 718-720.
@article{4b4c04b0af4d43a59a1bbf95da7f2c1a,
title = "DNA-catalyzed sequence-specific hydrolysis of DNA",
abstract = "Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and with a rate enhancement of 108 (possibly as high as 1014). The new DNA catalysts require both Mn2+ and Zn2+, which is noteworthy because many natural DNA nucleases are bimetallic protein enzymes.",
author = "Madhavaiah Chandra and Amit Sachdeva and Silverman, {Scott K}",
year = "2009",
month = "10",
doi = "10.1038/nchembio.201",
language = "English (US)",
volume = "5",
pages = "718--720",
journal = "Nature Chemical Biology",
issn = "1552-4450",
publisher = "Nature Publishing Group",
number = "10",

}

TY - JOUR

T1 - DNA-catalyzed sequence-specific hydrolysis of DNA

AU - Chandra, Madhavaiah

AU - Sachdeva, Amit

AU - Silverman, Scott K

PY - 2009/10

Y1 - 2009/10

N2 - Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and with a rate enhancement of 108 (possibly as high as 1014). The new DNA catalysts require both Mn2+ and Zn2+, which is noteworthy because many natural DNA nucleases are bimetallic protein enzymes.

AB - Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and with a rate enhancement of 108 (possibly as high as 1014). The new DNA catalysts require both Mn2+ and Zn2+, which is noteworthy because many natural DNA nucleases are bimetallic protein enzymes.

UR - http://www.scopus.com/inward/record.url?scp=70349327390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349327390&partnerID=8YFLogxK

U2 - 10.1038/nchembio.201

DO - 10.1038/nchembio.201

M3 - Article

C2 - 19684594

AN - SCOPUS:70349327390

VL - 5

SP - 718

EP - 720

JO - Nature Chemical Biology

JF - Nature Chemical Biology

SN - 1552-4450

IS - 10

ER -