DNA-catalyzed sequence-specific hydrolysis of DNA

Madhavaiah Chandra, Amit Sachdeva, Scott K. Silverman

Research output: Contribution to journalArticlepeer-review


Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and with a rate enhancement of 108 (possibly as high as 1014). The new DNA catalysts require both Mn2+ and Zn2+, which is noteworthy because many natural DNA nucleases are bimetallic protein enzymes.

Original languageEnglish (US)
Pages (from-to)718-720
Number of pages3
JournalNature chemical biology
Issue number10
StatePublished - Oct 2009

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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