DNA barcoding: A taxonomic point of view

Research output: Contribution to journalReview article

Abstract

A taxonomic perspective of deoxyribonucleic acid (DNA) barcoding is presented here with respect to its use within the freshwater science community. The goals and methods of DNA barcoding and the criticisms leveled at the procedure by the taxonomic community are explained. The major goal of DNA-barcoding efforts is to aid identification of specimens by matching sequences to a sequence library. This goal is achievable, but barcoding efforts must be coupled with a much wider sampling regime and should be done with the full cooperation and collaboration of the taxonomic community. Through collaborative efforts, taxonomists and barcode advocates can build capacity for taxonomy and sequence the vast number of specimens needed to build a truly comprehensive barcode library for use by benthic scientists. Other barcoding goals, such as species diagnosis and biodiversity surveys, are more problematic, but solutions are possible.

Original languageEnglish (US)
Pages (from-to)174-181
Number of pages8
JournalJournal of the North American Benthological Society
Volume30
Issue number1
DOIs
StatePublished - Mar 1 2011

Fingerprint

barcoding
DNA
biodiversity
sampling
DNA libraries
library
taxonomy

Keywords

  • DNA barcoding
  • aquatic insects
  • freshwater
  • macroinvertebrates

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

DNA barcoding : A taxonomic point of view. / Dewalt, R. Edward.

In: Journal of the North American Benthological Society, Vol. 30, No. 1, 01.03.2011, p. 174-181.

Research output: Contribution to journalReview article

@article{1fad6ed41a0f48d484a43df5b61f1b42,
title = "DNA barcoding: A taxonomic point of view",
abstract = "A taxonomic perspective of deoxyribonucleic acid (DNA) barcoding is presented here with respect to its use within the freshwater science community. The goals and methods of DNA barcoding and the criticisms leveled at the procedure by the taxonomic community are explained. The major goal of DNA-barcoding efforts is to aid identification of specimens by matching sequences to a sequence library. This goal is achievable, but barcoding efforts must be coupled with a much wider sampling regime and should be done with the full cooperation and collaboration of the taxonomic community. Through collaborative efforts, taxonomists and barcode advocates can build capacity for taxonomy and sequence the vast number of specimens needed to build a truly comprehensive barcode library for use by benthic scientists. Other barcoding goals, such as species diagnosis and biodiversity surveys, are more problematic, but solutions are possible.",
keywords = "DNA barcoding, aquatic insects, freshwater, macroinvertebrates",
author = "Dewalt, {R. Edward}",
year = "2011",
month = "3",
day = "1",
doi = "10.1899/10-021.1",
language = "English (US)",
volume = "30",
pages = "174--181",
journal = "Freshwater Science",
issn = "2161-9549",
publisher = "The Society for Freshwater Science",
number = "1",

}

TY - JOUR

T1 - DNA barcoding

T2 - A taxonomic point of view

AU - Dewalt, R. Edward

PY - 2011/3/1

Y1 - 2011/3/1

N2 - A taxonomic perspective of deoxyribonucleic acid (DNA) barcoding is presented here with respect to its use within the freshwater science community. The goals and methods of DNA barcoding and the criticisms leveled at the procedure by the taxonomic community are explained. The major goal of DNA-barcoding efforts is to aid identification of specimens by matching sequences to a sequence library. This goal is achievable, but barcoding efforts must be coupled with a much wider sampling regime and should be done with the full cooperation and collaboration of the taxonomic community. Through collaborative efforts, taxonomists and barcode advocates can build capacity for taxonomy and sequence the vast number of specimens needed to build a truly comprehensive barcode library for use by benthic scientists. Other barcoding goals, such as species diagnosis and biodiversity surveys, are more problematic, but solutions are possible.

AB - A taxonomic perspective of deoxyribonucleic acid (DNA) barcoding is presented here with respect to its use within the freshwater science community. The goals and methods of DNA barcoding and the criticisms leveled at the procedure by the taxonomic community are explained. The major goal of DNA-barcoding efforts is to aid identification of specimens by matching sequences to a sequence library. This goal is achievable, but barcoding efforts must be coupled with a much wider sampling regime and should be done with the full cooperation and collaboration of the taxonomic community. Through collaborative efforts, taxonomists and barcode advocates can build capacity for taxonomy and sequence the vast number of specimens needed to build a truly comprehensive barcode library for use by benthic scientists. Other barcoding goals, such as species diagnosis and biodiversity surveys, are more problematic, but solutions are possible.

KW - DNA barcoding

KW - aquatic insects

KW - freshwater

KW - macroinvertebrates

UR - http://www.scopus.com/inward/record.url?scp=79952261930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952261930&partnerID=8YFLogxK

U2 - 10.1899/10-021.1

DO - 10.1899/10-021.1

M3 - Review article

AN - SCOPUS:79952261930

VL - 30

SP - 174

EP - 181

JO - Freshwater Science

JF - Freshwater Science

SN - 2161-9549

IS - 1

ER -