DNA assembler method for construction of zeaxanthin-producing strains of saccharomyces cerevisiae

Zengyi Shao, Yunzi Luo, Huimin Zhao

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

DNA assembler enables design and rapid construction of biochemical pathways in a one-step fashion by exploitation of the in vivo homologous recombination mechanism in Saccharomyces cerevisiae. It has many applications in pathway engineering, metabolic engineering, combinatorial biology, and synthetic biology. Here we use the zeaxanthin biosynthetic pathway as an example to describe the key steps in the construction of pathways containing multiple genes using the DNA assembler approach. Methods for the construction of the clones, S. cerevisiae transformation, and zeaxanthin production and detection are shown.

Original languageEnglish (US)
Title of host publicationMicrobial Carotenoids From Fungi
Subtitle of host publicationMethods and Protocols
EditorsBarredo Jose-Luis
Pages251-262
Number of pages12
DOIs
StatePublished - Jul 27 2012

Publication series

NameMethods in Molecular Biology
Volume898
ISSN (Print)1064-3745

Keywords

  • DNA assembler
  • In vivo homologous recombination
  • Metabolic engineering
  • Pathway engineering
  • Synthetic biology
  • Zeaxanthin biosynthesis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Shao, Z., Luo, Y., & Zhao, H. (2012). DNA assembler method for construction of zeaxanthin-producing strains of saccharomyces cerevisiae. In B. Jose-Luis (Ed.), Microbial Carotenoids From Fungi: Methods and Protocols (pp. 251-262). (Methods in Molecular Biology; Vol. 898). https://doi.org/10.1007/978-1-61779-918-1_17