DNA and DNAzyme-mediated 2D colloidal assembly

Margaret H.S. Shyr, Daryl P. Wernette, Pierre Wiltzius, Yi Lu, Paul V. Braun

Research output: Contribution to journalArticlepeer-review

Abstract

DNA-mediated interactions present a significant opportunity for controlling colloidal self-assembly. Using microcontact printing to achieve spatial control of DNA-surface patterning and DNA-functionalized polystyrene colloids, we report that DNA hybridization can be utilized for sequence-specific reversible self-assembly of well-ordered 2D colloidal arrays. Two essential indicators of DNA-hybridization mediated assembly were confirmed; thermal reversibility and sequence specificity. The arrays melted at 50°C and reassembled when introduced to fresh colloid suspension, and sequence specificity with <1% nonspecific binding was confirmed using fluorescent polystyrene colloids. The real-time assembly of the colloids onto the periodically patterned substrate was monitored by simple laser diffraction to obtain assembly kinetics. Maximum surface coverage of DNA-mediated assembly was determined to be 0.593 for DNA-functionalized 100 nm polystyrene colloids, and 90% of the assembly was complete after 6.25 h of hybridization in 50 mM NaCl Tris buffer. We also demonstrate that DNAzymes, catalytic DNA molecules, can be incorporated into the design, and in the presence of 10 μM Pb2+, the hybridization-induced array assembly can be disrupted via DNAzyme activity.

Original languageEnglish (US)
Pages (from-to)8234-8240
Number of pages7
JournalJournal of the American Chemical Society
Volume130
Issue number26
DOIs
StatePublished - Jul 2 2008

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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