DNA amplification fingerprinting using arbitrary oligonucleotide primers

Gustavo Caetano-Anollés, Brant J. Bassam

Research output: Contribution to journalArticlepeer-review

Abstract

DNA amplification fingerprinting (DAF) is a strategy for genetic typing and mapping that uses one or more very short (≥5 nt) arbitrary oligonucleotides to direct the enzymatic amplification of discrete portions of a DNA template resulting in a spectrum of products characteristic of the DNA starting material. Polymorphisms from simple banding patterns are useful as genetic markers while more complex and informative patterns are suitable for DNA fingerprinting. The use of polyacrylamide gel electrophoresis and silver staining can adequately resolve the spectrum of DAF products into detailed and reproducible patterns.

Original languageEnglish (US)
Pages (from-to)189-200
Number of pages12
JournalApplied Biochemistry and Biotechnology
Volume42
Issue number2-3
DOIs
StatePublished - Aug 1 1993
Externally publishedYes

Keywords

  • DAF
  • DNA amplification
  • PCR
  • fingerprinting
  • primer-template interactions
  • single oligonucleotide primers

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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