Diversity of the Heme-Copper superfamily in archaea: Insights from genomics and structural modeling

James Hemp, Robert B. Gennis

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Recent advances in DNA sequencing technologies have provided unprecedented access into the diversity of the microbial world. Herein we use the comparative genomic analysis of microbial genomes and environmental metagenomes coupled with structural modelling to explore the diversity of aerobic respiration in Archaea. We focus on the heme-copper oxidoreductase superfamily which is responsible for catalyzing the terminal reaction in aerobic respiration - the reduction of molecular oxygen to water. Sequence analyses demonstrate that there are at least eight heme-copper oxygen reductase families: A-, B-, C-, D-, E-, F-, G-, and H-families. Interestingly, five of these oxygen reductase families (D-, E-, F-, G-, and H-families) are currently found exclusively in Archaea. We review the structural properties of all eight families focusing on the members found within Archaea. Structural modelling coupled with sequence analysis suggests that many of the oxygen reductases identified from thermophilic Archaea have modified proton channel properties compared to the currently studied mesophilic bacterial oxygen reductases. These structural differences may be due to adaptation to the specific environments in which these enzymes function. We conclude with a brief analysis of the phylogenetic distribution and evolution of Archaeal heme-copper oxygen reductases.

Original languageEnglish (US)
Title of host publicationBioenergetics
Subtitle of host publicationEnergy Conservation and Conversion
EditorsGünter Schäfer, Harvey Penefsky
Pages1-31
Number of pages31
DOIs
StatePublished - Jun 5 2008

Publication series

NameResults and Problems in Cell Differentiation
Volume45
ISSN (Print)0080-1844
ISSN (Electronic)1861-0412

Fingerprint

Archaea
Genomics
Heme
Copper
Oxidoreductases
Oxygen
Sequence Analysis
Respiration
Microbial Genome
Metagenome
DNA Sequence Analysis
Protons
Technology
Water
Enzymes

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Hemp, J., & Gennis, R. B. (2008). Diversity of the Heme-Copper superfamily in archaea: Insights from genomics and structural modeling. In G. Schäfer, & H. Penefsky (Eds.), Bioenergetics: Energy Conservation and Conversion (pp. 1-31). (Results and Problems in Cell Differentiation; Vol. 45). https://doi.org/10.1007/400_2007_046

Diversity of the Heme-Copper superfamily in archaea : Insights from genomics and structural modeling. / Hemp, James; Gennis, Robert B.

Bioenergetics: Energy Conservation and Conversion. ed. / Günter Schäfer; Harvey Penefsky. 2008. p. 1-31 (Results and Problems in Cell Differentiation; Vol. 45).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hemp, J & Gennis, RB 2008, Diversity of the Heme-Copper superfamily in archaea: Insights from genomics and structural modeling. in G Schäfer & H Penefsky (eds), Bioenergetics: Energy Conservation and Conversion. Results and Problems in Cell Differentiation, vol. 45, pp. 1-31. https://doi.org/10.1007/400_2007_046
Hemp J, Gennis RB. Diversity of the Heme-Copper superfamily in archaea: Insights from genomics and structural modeling. In Schäfer G, Penefsky H, editors, Bioenergetics: Energy Conservation and Conversion. 2008. p. 1-31. (Results and Problems in Cell Differentiation). https://doi.org/10.1007/400_2007_046
Hemp, James ; Gennis, Robert B. / Diversity of the Heme-Copper superfamily in archaea : Insights from genomics and structural modeling. Bioenergetics: Energy Conservation and Conversion. editor / Günter Schäfer ; Harvey Penefsky. 2008. pp. 1-31 (Results and Problems in Cell Differentiation).
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