TY - JOUR
T1 - Diversity and evolution of nitric oxide reduction in bacteria and archaea
AU - Murali, Ranjani
AU - Pace, Laura A.
AU - Sanford, Robert A.
AU - Ward, L. M.
AU - Lynes, Mackenzie M.
AU - Hatzenpichler, Roland
AU - Lingappa, Usha F.
AU - Fischer, Woodward W.
AU - Gennis, Robert B.
AU - Hemp, James
N1 - ACKNOWLEDGMENTS. We thank the NIH for funding support (grant# U12AB123456 to PI: R.B.G.). This research was also supported by funding from the Agouron Institute (W.W.F. and J.H.) and by the Community Science Project 507064 (PI: R.H.) under the Joint Genome Institute (https://ror.org/04xm1d337), which is a Department of Energy (DOE) Office of Science User Facility. Resources were also used at Office of Biological and Environmental Research of the US Department of Energy Atmospheric System Research Program Interagency Agreement grant DE-AC02-05CH11231 (JGI). Resources were used at Office of Biological and Environmental Research of the United States Department of Energy Atmospheric System Research Program Interagency Agreement grant DE-AC05-76RL01830 Environmental Molecular Sciences Laboratory (EMSL). We thank Sylvia Choi for providing pure ba3 oxygen reductase from T. thermophilus to use as a control for oxygen reductase assays and for heme extraction, Paige Sheridan for providing purified qNOR from Persephonella marina, Lici Schurig-Briccio for guidance in performing NOR assays with the Clark Electrode, and Peter Yau at the University of Illinois\u2019 Mass spectrometric facility for protein identification.We thank Alon Philosof and Connor Skennerton for valuable discussions on bioinfor-matics analysis. Finally, we would like to thank our reviewers for their valuable comments that led to the improvement of this manuscript.
PY - 2024/6/1
Y1 - 2024/6/1
N2 - Nitrous oxide is a potent greenhouse gas whose production is catalyzed by nitric oxide reductase (NOR) members of the heme-copper oxidoreductase (HCO) enzyme superfamily. We identified several previously uncharacterized HCO families, four of which (eNOR, sNOR, gNOR, and nNOR) appear to perform NO reduction. These families have novel active-site structures and several have conserved proton channels, suggesting that they might be able to couple NO reduction to energy conservation. We isolated and biochemically characterized a member of the eNOR family from the bacterium Rhodothermus marinus and found that it performs NO reduction. These recently identified NORs exhibited broad phylogenetic and environmental distributions, greatly expanding the diversity of microbes in nature capable of NO reduction. Phylogenetic analyses further demonstrated that NORs evolved multiple times independently from oxygen reductases, supporting the view that complete denitrification evolved after aerobic respiration.
AB - Nitrous oxide is a potent greenhouse gas whose production is catalyzed by nitric oxide reductase (NOR) members of the heme-copper oxidoreductase (HCO) enzyme superfamily. We identified several previously uncharacterized HCO families, four of which (eNOR, sNOR, gNOR, and nNOR) appear to perform NO reduction. These families have novel active-site structures and several have conserved proton channels, suggesting that they might be able to couple NO reduction to energy conservation. We isolated and biochemically characterized a member of the eNOR family from the bacterium Rhodothermus marinus and found that it performs NO reduction. These recently identified NORs exhibited broad phylogenetic and environmental distributions, greatly expanding the diversity of microbes in nature capable of NO reduction. Phylogenetic analyses further demonstrated that NORs evolved multiple times independently from oxygen reductases, supporting the view that complete denitrification evolved after aerobic respiration.
KW - Rhodothermus marinus
KW - aerobic denitrification
KW - denitrification
KW - heme-copper oxygen reductase
KW - nitric oxide reductase
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U2 - 10.1073/pnas.2316422121
DO - 10.1073/pnas.2316422121
M3 - Article
C2 - 38900790
AN - SCOPUS:85196908831
SN - 0027-8424
VL - 121
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 26
M1 - e2316422121
ER -