TY - JOUR
T1 - Diversity and evolution of a newly emerged North American Type 1 porcine arterivirus
T2 - Analysis of isolates collected between 1999 and 2004
AU - Fang, Y.
AU - Schneider, P.
AU - Zhang, W. P.
AU - Faaberg, K. S.
AU - Nelson, E. A.
AU - Rowland, Raymond Robert Richard
PY - 2007/5
Y1 - 2007/5
N2 - European-like Type 1 porcine reproductive and respiratory syndrome virus (PRRSV) isolates, known as North American (NA) Type 1 PRRSV, appeared in United States (U.S.) swine herds in 1999. Their diversity and evolution were studied over a five-year period by constructing phylogenetic trees using nsp2 and ORF5 sequences of 20 NA Type 1 isolates, including the only known isolate from Hawaii. All but two of the isolates possessed the same 51-nt deletion in nsp2, suggesting a clonal origin. Parsimony and distance analysis showed that viruses could be placed into two distinct sub-clades, which were similar for both nsp2 and ORF5. An incongruity between the two trees identified one isolate, 04-41, as the product of recombination. Recombination analysis using SimPlot identified a break point located downstream of the nsp2/3 junction. Results from this study suggest that NA Type 1 PRRSV in the U.S. is a well-established and rapidly evolving group. However, the forces driving genetic diversity and separation are complex and remain to be elucidated.
AB - European-like Type 1 porcine reproductive and respiratory syndrome virus (PRRSV) isolates, known as North American (NA) Type 1 PRRSV, appeared in United States (U.S.) swine herds in 1999. Their diversity and evolution were studied over a five-year period by constructing phylogenetic trees using nsp2 and ORF5 sequences of 20 NA Type 1 isolates, including the only known isolate from Hawaii. All but two of the isolates possessed the same 51-nt deletion in nsp2, suggesting a clonal origin. Parsimony and distance analysis showed that viruses could be placed into two distinct sub-clades, which were similar for both nsp2 and ORF5. An incongruity between the two trees identified one isolate, 04-41, as the product of recombination. Recombination analysis using SimPlot identified a break point located downstream of the nsp2/3 junction. Results from this study suggest that NA Type 1 PRRSV in the U.S. is a well-established and rapidly evolving group. However, the forces driving genetic diversity and separation are complex and remain to be elucidated.
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U2 - 10.1007/s00705-007-0936-y
DO - 10.1007/s00705-007-0936-y
M3 - Article
C2 - 17323198
AN - SCOPUS:34247464685
SN - 0304-8608
VL - 152
SP - 1009
EP - 1017
JO - Archives of Virology
JF - Archives of Virology
IS - 5
ER -