@article{4f01b7fc6e5444149cd75291ed2c2b33,
title = "Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis",
abstract = "In the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an extrachromosomal marker (mar) element. All confirmed carrier hiPSCs and differentiated neural progenitor cell lines were found to be mosaic. We caution that mar elements may be difficult to functionally evaluate in hiPSC cultures using currently available methods, as it is difficult to distinguish cells with and without mar elements in live mosaic cultures.",
keywords = "9p24.1, bipolar disorder, human induced pluripotent stem cell, isogenic, marker chromosome, mosaic, neural progenitor cell, schizophrenia",
author = "Julia TCW and Carvalho, {Claudia M.B.} and Bo Yuan and Shen Gu and Altheimer, {Alyssa N.} and Shane McCarthy and Dheeraj Malhotra and Jonathan Sebat and Siegel, {Arthur J.} and Uwe Rudolph and Lupski, {James R.} and Levy, {Deborah L.} and Brennand, {Kristen J.}",
note = "Funding Information: Alison Goate kindly offered use of sequencing machine and reagents for fingerprinting of HFs and hiPSCs. Jano Roze assisted in the expansion of some hiPSC lines. All karyotype and FISH analyses were performed by the Cytogenetic Laboratory of WiCell Research Institute. K.J.B. is a New York Stem Cell Foundation - Robertson Investigator. This work was supported in part by NIH grants R01 MH101454 (K.J.B.), R21 MH097470 (D.L.L.), R21 MH1057321 (D.L.L.), RO1NS058529 (J.R.L.), R21 MH104505 (U.R.), New York Stem Cell Foundation (K.J.B.), Brain and Behavior Young Investigator Grant (K.J.B.), the Ellison Foundation (D.L.L., K.J.B., J.R.L., C.M.B.C.), Anonymous Foundation (D.L.L.), the Carmela and Menachem Abraham Fund (D.L.L.), and Team Daniel (D.L.L.). Publisher Copyright: {\textcopyright} 2017 The Authors",
year = "2017",
month = mar,
day = "14",
doi = "10.1016/j.stemcr.2017.01.010",
language = "English (US)",
volume = "8",
pages = "519--528",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "3",
}