Distribution of horseradish peroxidase activity in horseradish plants

Mosbah M. Kushad, Mohammed Guidera, Anthony D. Bratsch

Research output: Contribution to journalArticlepeer-review


Thirty horseradish (Armoracia rusticana Gaertn., Mey., and Scherb.) cultivars from eight countries in Europe and North America and from advanced lines developed at the Univ. of Illinois were evaluated for horseradish peroxidase (HRP; electrical conductivity, donor: hydrogen peroxide oxido-reductase) activity. Nearly 86% of the activity was present in the taproot and lateral roots and 14% in the leaf petiole, but there was no activity in the leaf blade. The 30 cultivars were divided into three groups with high (eight cultivars), medium (13 cultivars), and low (nine cultivars) activities [11.58 to 16.97, 7.19 to 9.79, and 2.88 to 6.91 μmol·min-1·g-1 fresh weight (FW), respectively]. The cultivars with the highest activity were 819-A from the Illinois and 810-A from Switzerland with 16.97 and 16.67 μmol·min-1·g-1 FW, respectively. The cultivar with the lowest HRP activity was 244-A from the United States with 2.88 μmol·min-1·g-1 FW. Cultivar 819-A also had the highest protein concentration (4.92 mg·g-1 FW). When HRP activity was expressed per milligrams of protein, cultivar 167-A, also known as 'Bohemian', had the highest activity and cultivar 244-A had the lowest (5.35 and 0.83 μmol·min-1·mg-1 protein, respectively).

Original languageEnglish (US)
Pages (from-to)127-129
Number of pages3
Issue number1
StatePublished - 1999


  • Aromoracia rusticana
  • Decontamination
  • Enzyme
  • Health
  • Protein

ASJC Scopus subject areas

  • Horticulture


Dive into the research topics of 'Distribution of horseradish peroxidase activity in horseradish plants'. Together they form a unique fingerprint.

Cite this