Distinct transcriptional profiles of ozone stress in soybean (Glycine max) flowers and pods

Courtney P. Leisner, Ray Ming, Elizabeth A. Ainsworth

Research output: Contribution to journalArticle

Abstract

Background: Tropospheric ozone (O3) is a secondary air pollutant and anthropogenic greenhouse gas. Concentrations of tropospheric O3 ([O3] have more than doubled since the Industrial Revolution, and are high enough to damage plant productivity. Soybean (Glycine max L. Merr.) is the world's most important legume crop and is sensitive to O3. Current ground-level [O3] are estimated to reduce global soybean yields by 6% to 16%. In order to understand transcriptional mechanisms of yield loss in soybean, we examined the transcriptome of soybean flower and pod tissues exposed to elevated [O3] using RNA-Sequencing. Results: Elevated [O3] elicited a strong transcriptional response in flower and pod tissues, with increased expression of genes involved in signaling in both tissues. Flower tissues also responded to elevated [O3] by increasing expression of genes encoding matrix metalloproteinases (MMPs). MMPs are zinc- and calcium-dependent endopeptidases that have roles in programmed cell death, senescence and stress response in plants. Pod tissues responded to elevated [O3] by increasing expression of xyloglucan endotransglucosylase/hydrolase genes, which may be involved with increased pod dehiscence in elevated [O3]. Conclusions: This study established that gene expression in reproductive tissues of soybean are impacted by elevated [O3], and flowers and pods have distinct transcriptomic responses to elevated [O3].

Original languageEnglish (US)
Article number335
JournalBMC Plant Biology
Volume14
Issue number1
DOIs
StatePublished - Jan 1 2014

Fingerprint

ozone
pods
Glycine max
soybeans
flowers
metalloproteinases
gene expression
xyloglucan:xyloglucosyl transferase
dehiscence
plant damage
greenhouse gases
hydrolases
transcriptomics
transcriptome
tissues
stress response
proteinases
apoptosis
sequence analysis
legumes

Keywords

  • Cell wall modification
  • Glycine max
  • Matrix metalloproteinases
  • Oxidative stress
  • RNA-Sequencing

ASJC Scopus subject areas

  • Plant Science

Cite this

Distinct transcriptional profiles of ozone stress in soybean (Glycine max) flowers and pods. / Leisner, Courtney P.; Ming, Ray; Ainsworth, Elizabeth A.

In: BMC Plant Biology, Vol. 14, No. 1, 335, 01.01.2014.

Research output: Contribution to journalArticle

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