Distending stress of the cytoskeleton is a key determinant of cell rheological behavior

Noah Rosenblatt, Shaohua Hu, Jianxin Chen, Ning Wang, Dimitrije Stamenović

Research output: Contribution to journalArticle

Abstract

One fundamental question in cell biology is what determines rheological properties of living cells. If the cytoskeletal distending stress is a key determinant of cell rheology, then modulating this stress by cell stretching should have a major effect on cell rheological properties. If not, then other mechanisms must play a major role. We developed a stretchable cell culture device that could rapidly stretch cells and thus generate passive mechanical stress within the cytoskeleton. This device was placed inside a magnetic cytometry system to measure the effect of stretching on rheological properties of cultured human airway smooth muscle cells. A gradual increase in cell distension caused a systematic increase in cell dynamic stiffness in a manner which was consistent with earlier observations where the active component of the distending stress was modulated pharmacologically. These findings provide strong evidence that the cytoskeletal distending stress is a key determinant of cell rheological properties.

Original languageEnglish (US)
Pages (from-to)617-622
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume321
Issue number3
DOIs
StatePublished - Aug 27 2004
Externally publishedYes

Keywords

  • Cell stretch
  • Cytoskeleton
  • Distending stress
  • Dynamic stiffness
  • Magnetic oscillatory cytometry
  • Power law
  • Rheology

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Distending stress of the cytoskeleton is a key determinant of cell rheological behavior'. Together they form a unique fingerprint.

  • Cite this