Abstract
To evaluate the role of polyamines in mediating the effect of estrogen on growth of the rat uterus, we examined the effect of an irreversible inhibitor of ornithine decarboxylase (ODC), 5α-difluoromethylornithine (DFMO), on estrogen-stimulated uterine growth. Estradiol alone evoked two major surges of ODC activity at 5 h and at 16 h, and 2-fold increases in the concentration of spermidine in the uterus. When DFMO was administered to either intact or ovariectomized immature (20 day-old) rats for 2 or 6 days prior to and then during the 1 or 3 days of estradiol treatment, uterine ODC activity was completely suppressed as was the associated increase in spermidine concentration, but the magnitude of uterine growth stimulated by estradiol was equally great in the presence or absence of DFMO. These observations suggest that although uterine growth stimulation by estrogen is normally accompanied by marked increases in ODC activity and spermidine accumulation, these changes are not essential for hormonally regulated uterine cell proliferation in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1186-1193 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 122 |
| Issue number | 3 |
| DOIs | |
| State | Published - Aug 16 1984 |
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ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Dissociated regulation of growth and ornithine decarboxylase activity by estrogen in the rat uterus. / Rorke, Ellen A.; Katzenellenbogen, Benita S.
In: Biochemical and Biophysical Research Communications, Vol. 122, No. 3, 16.08.1984, p. 1186-1193.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dissociated regulation of growth and ornithine decarboxylase activity by estrogen in the rat uterus
AU - Rorke, Ellen A.
AU - Katzenellenbogen, Benita S.
PY - 1984/8/16
Y1 - 1984/8/16
N2 - To evaluate the role of polyamines in mediating the effect of estrogen on growth of the rat uterus, we examined the effect of an irreversible inhibitor of ornithine decarboxylase (ODC), 5α-difluoromethylornithine (DFMO), on estrogen-stimulated uterine growth. Estradiol alone evoked two major surges of ODC activity at 5 h and at 16 h, and 2-fold increases in the concentration of spermidine in the uterus. When DFMO was administered to either intact or ovariectomized immature (20 day-old) rats for 2 or 6 days prior to and then during the 1 or 3 days of estradiol treatment, uterine ODC activity was completely suppressed as was the associated increase in spermidine concentration, but the magnitude of uterine growth stimulated by estradiol was equally great in the presence or absence of DFMO. These observations suggest that although uterine growth stimulation by estrogen is normally accompanied by marked increases in ODC activity and spermidine accumulation, these changes are not essential for hormonally regulated uterine cell proliferation in vivo.
AB - To evaluate the role of polyamines in mediating the effect of estrogen on growth of the rat uterus, we examined the effect of an irreversible inhibitor of ornithine decarboxylase (ODC), 5α-difluoromethylornithine (DFMO), on estrogen-stimulated uterine growth. Estradiol alone evoked two major surges of ODC activity at 5 h and at 16 h, and 2-fold increases in the concentration of spermidine in the uterus. When DFMO was administered to either intact or ovariectomized immature (20 day-old) rats for 2 or 6 days prior to and then during the 1 or 3 days of estradiol treatment, uterine ODC activity was completely suppressed as was the associated increase in spermidine concentration, but the magnitude of uterine growth stimulated by estradiol was equally great in the presence or absence of DFMO. These observations suggest that although uterine growth stimulation by estrogen is normally accompanied by marked increases in ODC activity and spermidine accumulation, these changes are not essential for hormonally regulated uterine cell proliferation in vivo.
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U2 - 10.1016/0006-291X(84)91217-8
DO - 10.1016/0006-291X(84)91217-8
M3 - Article
C2 - 6433909
AN - SCOPUS:0021238909
VL - 122
SP - 1186
EP - 1193
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -