TY - GEN
T1 - Dissecting the influences of combinatorial microenvironments on hepatic stellate cell phenotype and behavior
AU - Jain, I.
AU - Brougham-Cook, A.
AU - Kukla, D.
AU - Khetani, S.
AU - Underhill, Gregory H
PY - 2019/1/1
Y1 - 2019/1/1
N2 -
Statement of Purpose: Non-alcoholic steatohepatitis (NASH) is an emerging epidemic of liver disease and the basis for a rising incidence of fibrosis and hepatocellular carcinoma
1
. Recently, hepatic stellate cells (HSCs) have been identified as the primary drivers of liver fibrosis
2
. Changes in the composition of the liver microenvironment during fibrosis results in a complex crosstalk of extracellular cues that promotes the activation, or differentiation, of HSCs from a quiescent, vitamin A storing phenotype to a myofibroblastic phenotype. While these chemo-mechanical signaling processes are well understood in rodent HSC models, very little is known about extracellular influences on HSC activation in humans. Here, we have utilized a high-throughput cellular microarray platform to dissect the cooperative influences of defined combinations of extracellular matrix (ECM) proteins and substrate stiffnesses on human HSC phenotype and behavior
3
.
AB -
Statement of Purpose: Non-alcoholic steatohepatitis (NASH) is an emerging epidemic of liver disease and the basis for a rising incidence of fibrosis and hepatocellular carcinoma
1
. Recently, hepatic stellate cells (HSCs) have been identified as the primary drivers of liver fibrosis
2
. Changes in the composition of the liver microenvironment during fibrosis results in a complex crosstalk of extracellular cues that promotes the activation, or differentiation, of HSCs from a quiescent, vitamin A storing phenotype to a myofibroblastic phenotype. While these chemo-mechanical signaling processes are well understood in rodent HSC models, very little is known about extracellular influences on HSC activation in humans. Here, we have utilized a high-throughput cellular microarray platform to dissect the cooperative influences of defined combinations of extracellular matrix (ECM) proteins and substrate stiffnesses on human HSC phenotype and behavior
3
.
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UR - http://www.scopus.com/inward/citedby.url?scp=85065413545&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85065413545
T3 - Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
BT - Society for Biomaterials Annual Meeting and Exposition 2019
PB - Society for Biomaterials
T2 - 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
Y2 - 3 April 2019 through 6 April 2019
ER -