TY - GEN
T1 - Dissecting the influences of combinatorial microenvironments on hepatic stellate cell phenotype and behavior
AU - Jain, I.
AU - Brougham-Cook, A.
AU - Kukla, D.
AU - Khetani, S.
AU - Underhill, G.
N1 - Publisher Copyright:
© 2019 Omnipress - All rights reserved.
PY - 2019
Y1 - 2019
N2 - Statement of Purpose: Non-alcoholic steatohepatitis (NASH) is an emerging epidemic of liver disease and the basis for a rising incidence of fibrosis and hepatocellular carcinoma1. Recently, hepatic stellate cells (HSCs) have been identified as the primary drivers of liver fibrosis2. Changes in the composition of the liver microenvironment during fibrosis results in a complex crosstalk of extracellular cues that promotes the activation, or differentiation, of HSCs from a quiescent, vitamin A storing phenotype to a myofibroblastic phenotype. While these chemo-mechanical signaling processes are well understood in rodent HSC models, very little is known about extracellular influences on HSC activation in humans. Here, we have utilized a high-throughput cellular microarray platform to dissect the cooperative influences of defined combinations of extracellular matrix (ECM) proteins and substrate stiffnesses on human HSC phenotype and behavior3.
AB - Statement of Purpose: Non-alcoholic steatohepatitis (NASH) is an emerging epidemic of liver disease and the basis for a rising incidence of fibrosis and hepatocellular carcinoma1. Recently, hepatic stellate cells (HSCs) have been identified as the primary drivers of liver fibrosis2. Changes in the composition of the liver microenvironment during fibrosis results in a complex crosstalk of extracellular cues that promotes the activation, or differentiation, of HSCs from a quiescent, vitamin A storing phenotype to a myofibroblastic phenotype. While these chemo-mechanical signaling processes are well understood in rodent HSC models, very little is known about extracellular influences on HSC activation in humans. Here, we have utilized a high-throughput cellular microarray platform to dissect the cooperative influences of defined combinations of extracellular matrix (ECM) proteins and substrate stiffnesses on human HSC phenotype and behavior3.
UR - http://www.scopus.com/inward/record.url?scp=85065413545&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85065413545&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85065413545
T3 - Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
SP - 395
BT - Society for Biomaterials Annual Meeting and Exposition 2019
PB - Society for Biomaterials
T2 - 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
Y2 - 3 April 2019 through 6 April 2019
ER -