Dissecting the influences of combinatorial microenvironments on hepatic stellate cell phenotype and behavior

I. Jain, A. Brougham-Cook, D. Kukla, S. Khetani, Gregory H Underhill

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: Non-alcoholic steatohepatitis (NASH) is an emerging epidemic of liver disease and the basis for a rising incidence of fibrosis and hepatocellular carcinoma 1 . Recently, hepatic stellate cells (HSCs) have been identified as the primary drivers of liver fibrosis 2 . Changes in the composition of the liver microenvironment during fibrosis results in a complex crosstalk of extracellular cues that promotes the activation, or differentiation, of HSCs from a quiescent, vitamin A storing phenotype to a myofibroblastic phenotype. While these chemo-mechanical signaling processes are well understood in rodent HSC models, very little is known about extracellular influences on HSC activation in humans. Here, we have utilized a high-throughput cellular microarray platform to dissect the cooperative influences of defined combinations of extracellular matrix (ECM) proteins and substrate stiffnesses on human HSC phenotype and behavior 3 .

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

Fingerprint

Hepatic Stellate Cells
Phenotype
Liver
Fibrosis
Mechanical Phenomena
Chemical activation
Extracellular Matrix Proteins
Vitamins
Fatty Liver
Microarrays
Crosstalk
Vitamin A
Liver Cirrhosis
Cues
Liver Diseases
Hepatocellular Carcinoma
Rodentia
Stiffness
Throughput
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

Cite this

Jain, I., Brougham-Cook, A., Kukla, D., Khetani, S., & Underhill, G. H. (2019). Dissecting the influences of combinatorial microenvironments on hepatic stellate cell phenotype and behavior. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.

Dissecting the influences of combinatorial microenvironments on hepatic stellate cell phenotype and behavior. / Jain, I.; Brougham-Cook, A.; Kukla, D.; Khetani, S.; Underhill, Gregory H.

Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Jain, I, Brougham-Cook, A, Kukla, D, Khetani, S & Underhill, GH 2019, Dissecting the influences of combinatorial microenvironments on hepatic stellate cell phenotype and behavior. in Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium, vol. 40, Society for Biomaterials, 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence, Seattle, United States, 4/3/19.
Jain I, Brougham-Cook A, Kukla D, Khetani S, Underhill GH. Dissecting the influences of combinatorial microenvironments on hepatic stellate cell phenotype and behavior. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials. 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium).
Jain, I. ; Brougham-Cook, A. ; Kukla, D. ; Khetani, S. ; Underhill, Gregory H. / Dissecting the influences of combinatorial microenvironments on hepatic stellate cell phenotype and behavior. Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium).
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