Dissecting the functional domains of a nonenveloped virus membrane penetration peptide

Manidipa Banerjee, Reza Khayat, Hanna E. Walukiewicz, Amy L. Odegard, Anette Schneemann, John E. Johnson

Research output: Contribution to journalArticlepeer-review

Abstract

Recent studies have established that several nonenveloped viruses utilize virus-encoded lytic peptides for host membrane disruption. We investigated this mechanism with the "gamma" peptide of the insect virus Flock House virus (FHV). We demonstrate that the C terminus of gamma is essential for membrane disruption in vitro and the rescue of immature virus infectivity in vivo, and the amphipathic N terminus of gamma alone is not sufficient. We also show that deletion of the C-terminal domain disrupts icosahedral ordering of the amphipathic helices of gamma in the virus. Our results have broad implications for understanding membrane lysis during nonenveloped virus entry.

Original languageEnglish (US)
Pages (from-to)6929-6933
Number of pages5
JournalJournal of virology
Volume83
Issue number13
DOIs
StatePublished - Jul 2009

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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