Abstract

We used quantitative phase imaging to measure the dispersion relation, i.e. decay rate vs. spatial mode, associated with mass transport in live cells. This approach applies equally well to both discrete and continuous mass distributions without the need for particle tracking. From the quadratic experimental curve specific to diffusion, we extracted the diffusion coefficient as the only fitting parameter. The linear portion of the dispersion relation reveals the deterministic component of the intracellular transport. Our data show a universal behavior where the intracellular transport is diffusive at small scales and deterministic at large scales. Measurements by our method and particle tracking show that, on average, the mass transport in the nucleus is slower than in the cytoplasm.

Original languageEnglish (US)
Pages (from-to)20571-20579
Number of pages9
JournalOptics Express
Volume19
Issue number21
DOIs
StatePublished - Oct 10 2011

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cytoplasm
mass distribution
spectroscopy
decay rates
diffusion coefficient
nuclei
curves
cells

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Dispersion-relation phase spectroscopy of intracellular transport. / Wang, Ru; Wang, Zhuo; Millet, Larry; Gillette, Martha U.; Levine, A. J.; Popescu, Gabriel.

In: Optics Express, Vol. 19, No. 21, 10.10.2011, p. 20571-20579.

Research output: Contribution to journalArticle

Wang, Ru ; Wang, Zhuo ; Millet, Larry ; Gillette, Martha U. ; Levine, A. J. ; Popescu, Gabriel. / Dispersion-relation phase spectroscopy of intracellular transport. In: Optics Express. 2011 ; Vol. 19, No. 21. pp. 20571-20579.
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