Abstract
We used quantitative phase imaging to measure the dispersion relation, i.e. decay rate vs. spatial mode, associated with mass transport in live cells. This approach applies equally well to both discrete and continuous mass distributions without the need for particle tracking. From the quadratic experimental curve specific to diffusion, we extracted the diffusion coefficient as the only fitting parameter. The linear portion of the dispersion relation reveals the deterministic component of the intracellular transport. Our data show a universal behavior where the intracellular transport is diffusive at small scales and deterministic at large scales. Measurements by our method and particle tracking show that, on average, the mass transport in the nucleus is slower than in the cytoplasm.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 20571-20579 |
| Number of pages | 9 |
| Journal | Optics Express |
| Volume | 19 |
| Issue number | 21 |
| DOIs | |
| State | Published - Oct 10 2011 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Dispersion-relation phase spectroscopy of intracellular transport. / Wang, Ru; Wang, Zhuo; Millet, Larry; Gillette, Martha U.; Levine, A. J.; Popescu, Gabriel.
In: Optics Express, Vol. 19, No. 21, 10.10.2011, p. 20571-20579.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dispersion-relation phase spectroscopy of intracellular transport
AU - Wang, Ru
AU - Wang, Zhuo
AU - Millet, Larry
AU - Gillette, Martha U.
AU - Levine, A. J.
AU - Popescu, Gabriel
PY - 2011/10/10
Y1 - 2011/10/10
N2 - We used quantitative phase imaging to measure the dispersion relation, i.e. decay rate vs. spatial mode, associated with mass transport in live cells. This approach applies equally well to both discrete and continuous mass distributions without the need for particle tracking. From the quadratic experimental curve specific to diffusion, we extracted the diffusion coefficient as the only fitting parameter. The linear portion of the dispersion relation reveals the deterministic component of the intracellular transport. Our data show a universal behavior where the intracellular transport is diffusive at small scales and deterministic at large scales. Measurements by our method and particle tracking show that, on average, the mass transport in the nucleus is slower than in the cytoplasm.
AB - We used quantitative phase imaging to measure the dispersion relation, i.e. decay rate vs. spatial mode, associated with mass transport in live cells. This approach applies equally well to both discrete and continuous mass distributions without the need for particle tracking. From the quadratic experimental curve specific to diffusion, we extracted the diffusion coefficient as the only fitting parameter. The linear portion of the dispersion relation reveals the deterministic component of the intracellular transport. Our data show a universal behavior where the intracellular transport is diffusive at small scales and deterministic at large scales. Measurements by our method and particle tracking show that, on average, the mass transport in the nucleus is slower than in the cytoplasm.
UR - http://www.scopus.com/inward/record.url?scp=80052539530&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052539530&partnerID=8YFLogxK
U2 - 10.1364/OE.19.020571
DO - 10.1364/OE.19.020571
M3 - Article
C2 - 21997064
AN - SCOPUS:80052539530
VL - 19
SP - 20571
EP - 20579
JO - Optics Express
JF - Optics Express
SN - 1094-4087
IS - 21
ER -