Disease modeling and gene therapy of copper storage disease in canine hepatic organoids

Sathidpak Nantasanti, Bart Spee, Hedwig S. Kruitwagen, Chen Chen, Niels Geijsen, Loes A. Oosterhoff, Monique E. Van Wolferen, Nicolas Pelaez, Hille Fieten, Richard W. Wubbolts, Guy C. Grinwis, Jefferson Chan, Meritxell Huch, Robert R.G. Vries, Hans Clevers, Alain De Bruin, Jan Rothuizen, Louis C. Penning, Baukje A. Schotanus

Research output: Contribution to journalArticle

Abstract

The recent development of 3D-liver stem cell cultures (hepatic organoids) opens up new avenues for gene and/or stem cell therapy to treat liver disease. To test safety and efficacy, a relevant large animal model is essential but not yet established. Because of its shared pathologies and disease pathways, the dog is considered the best model for human liver disease. Here we report the establishment of a long-term canine hepatic organoid culture allowing undifferentiated expansion of progenitor cells that can be differentiated toward functional hepatocytes. We show that cultures can be initiated from fresh and frozen liver tissues using Tru-Cut or fine-needle biopsies. The use of Wnt agonists proved important for canine organoid proliferation and inhibition of differentiation. Finally, we demonstrate that successful gene supplementation in hepatic organoids of COMMD1-deficient dogs restores function and can be an effective means to cure copper storage disease.

Original languageEnglish (US)
Pages (from-to)895-907
Number of pages13
JournalStem Cell Reports
Volume5
Issue number5
DOIs
StatePublished - Nov 10 2015

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

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  • Cite this

    Nantasanti, S., Spee, B., Kruitwagen, H. S., Chen, C., Geijsen, N., Oosterhoff, L. A., Van Wolferen, M. E., Pelaez, N., Fieten, H., Wubbolts, R. W., Grinwis, G. C., Chan, J., Huch, M., Vries, R. R. G., Clevers, H., De Bruin, A., Rothuizen, J., Penning, L. C., & Schotanus, B. A. (2015). Disease modeling and gene therapy of copper storage disease in canine hepatic organoids. Stem Cell Reports, 5(5), 895-907. https://doi.org/10.1016/j.stemcr.2015.09.002