Discrete frequency infrared imaging using quantum cascade lasers for biological tissue analysis

Kevin Yeh, Rohit Bhargava

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Infrared (IR) spectroscopic imaging is an emerging modality for biological tissue analysis that has traditionally employed an interferometer for spectral discrimination. Recent technology developments have made discrete frequency sources, both lasers and filters, practical for imaging. The use of quantum cascade lasers in particular, presents new opportunities as well as challenges. Here we describe results from a novel point scanning confocal IR microscope and demonstrate the performance imaging several important spectral bands of lung tissue. Results show the possibility of discrete frequency (DF) absorbance measurements with RMS noise levels down to 0.34 mAU in 0.25 ms.

Original languageEnglish (US)
Title of host publicationBiomedical Vibrational Spectroscopy 2016
Subtitle of host publicationAdvances in Research and Industry
EditorsAnita Mahadevan-Jansen, Wolfgang Petrich
PublisherSPIE
ISBN (Electronic)9781628419382
DOIs
StatePublished - Jan 1 2016
EventBiomedical Vibrational Spectroscopy 2016: Advances in Research and Industry - San Francisco, United States
Duration: Feb 13 2016Feb 14 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9704
ISSN (Print)1605-7422

Other

OtherBiomedical Vibrational Spectroscopy 2016: Advances in Research and Industry
CountryUnited States
CitySan Francisco
Period2/13/162/14/16

Fingerprint

Semiconductor Lasers
Quantum cascade lasers
Infrared imaging
quantum cascade lasers
Tissue
Imaging techniques
frequency measurement
spectral bands
lungs
Infrared radiation
discrimination
Noise
emerging
Lasers
interferometers
microscopes
filters
Interferometers
Lung
scanning

Keywords

  • Mid-infrared spectroscopy
  • Quantum Cascade Laser (QCL)
  • chemical imaging
  • discrete frequency infrared (DF-IR)
  • microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Yeh, K., & Bhargava, R. (2016). Discrete frequency infrared imaging using quantum cascade lasers for biological tissue analysis. In A. Mahadevan-Jansen, & W. Petrich (Eds.), Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry [970406] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9704). SPIE. https://doi.org/10.1117/12.2230003

Discrete frequency infrared imaging using quantum cascade lasers for biological tissue analysis. / Yeh, Kevin; Bhargava, Rohit.

Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry. ed. / Anita Mahadevan-Jansen; Wolfgang Petrich. SPIE, 2016. 970406 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9704).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yeh, K & Bhargava, R 2016, Discrete frequency infrared imaging using quantum cascade lasers for biological tissue analysis. in A Mahadevan-Jansen & W Petrich (eds), Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry., 970406, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9704, SPIE, Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry, San Francisco, United States, 2/13/16. https://doi.org/10.1117/12.2230003
Yeh K, Bhargava R. Discrete frequency infrared imaging using quantum cascade lasers for biological tissue analysis. In Mahadevan-Jansen A, Petrich W, editors, Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry. SPIE. 2016. 970406. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2230003
Yeh, Kevin ; Bhargava, Rohit. / Discrete frequency infrared imaging using quantum cascade lasers for biological tissue analysis. Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry. editor / Anita Mahadevan-Jansen ; Wolfgang Petrich. SPIE, 2016. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
@inproceedings{ec68d4c05e1243ca875f0db0ab7d859c,
title = "Discrete frequency infrared imaging using quantum cascade lasers for biological tissue analysis",
abstract = "Infrared (IR) spectroscopic imaging is an emerging modality for biological tissue analysis that has traditionally employed an interferometer for spectral discrimination. Recent technology developments have made discrete frequency sources, both lasers and filters, practical for imaging. The use of quantum cascade lasers in particular, presents new opportunities as well as challenges. Here we describe results from a novel point scanning confocal IR microscope and demonstrate the performance imaging several important spectral bands of lung tissue. Results show the possibility of discrete frequency (DF) absorbance measurements with RMS noise levels down to 0.34 mAU in 0.25 ms.",
keywords = "Mid-infrared spectroscopy, Quantum Cascade Laser (QCL), chemical imaging, discrete frequency infrared (DF-IR), microscopy",
author = "Kevin Yeh and Rohit Bhargava",
year = "2016",
month = "1",
day = "1",
doi = "10.1117/12.2230003",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Anita Mahadevan-Jansen and Wolfgang Petrich",
booktitle = "Biomedical Vibrational Spectroscopy 2016",

}

TY - GEN

T1 - Discrete frequency infrared imaging using quantum cascade lasers for biological tissue analysis

AU - Yeh, Kevin

AU - Bhargava, Rohit

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Infrared (IR) spectroscopic imaging is an emerging modality for biological tissue analysis that has traditionally employed an interferometer for spectral discrimination. Recent technology developments have made discrete frequency sources, both lasers and filters, practical for imaging. The use of quantum cascade lasers in particular, presents new opportunities as well as challenges. Here we describe results from a novel point scanning confocal IR microscope and demonstrate the performance imaging several important spectral bands of lung tissue. Results show the possibility of discrete frequency (DF) absorbance measurements with RMS noise levels down to 0.34 mAU in 0.25 ms.

AB - Infrared (IR) spectroscopic imaging is an emerging modality for biological tissue analysis that has traditionally employed an interferometer for spectral discrimination. Recent technology developments have made discrete frequency sources, both lasers and filters, practical for imaging. The use of quantum cascade lasers in particular, presents new opportunities as well as challenges. Here we describe results from a novel point scanning confocal IR microscope and demonstrate the performance imaging several important spectral bands of lung tissue. Results show the possibility of discrete frequency (DF) absorbance measurements with RMS noise levels down to 0.34 mAU in 0.25 ms.

KW - Mid-infrared spectroscopy

KW - Quantum Cascade Laser (QCL)

KW - chemical imaging

KW - discrete frequency infrared (DF-IR)

KW - microscopy

UR - http://www.scopus.com/inward/record.url?scp=84973333162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973333162&partnerID=8YFLogxK

U2 - 10.1117/12.2230003

DO - 10.1117/12.2230003

M3 - Conference contribution

AN - SCOPUS:84973333162

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Biomedical Vibrational Spectroscopy 2016

A2 - Mahadevan-Jansen, Anita

A2 - Petrich, Wolfgang

PB - SPIE

ER -