Discovery of novel bacterial queuine salvage enzymes and pathways in human pathogens

Yifeng Yuan, Rémi Zallot, Tyler L. Grove, Daniel J. Payan, Isabelle Martin-Verstraete, Sara Šepić, Seetharamsingh Balamkundu, Ramesh Neelakandan, Vinod K. Gadi, Chuan Fa Liu, Manal A. Swairjo, Peter C. Dedon, Steven C. Almo, John Alan Gerlt, Valérie de Crécy-Lagard

Research output: Contribution to journalArticle

Abstract

Queuosine (Q) is a complex tRNA modification widespread in eukaryotes and bacteria that contributes to the efficiency and accuracy of protein synthesis. Eukaryotes are not capable of Q synthesis and rely on salvage of the queuine base (q) as a Q precursor. While many bacteria are capable of Q de novo synthesis, salvage of the prokaryotic Q precursors preQ0 and preQ1 also occurs. With the exception of Escherichia coli YhhQ, shown to transport preQ0 and preQ1, the enzymes and transporters involved in Q salvage and recycling have not been well described. We discovered and characterized 2 Q salvage pathways present in many pathogenic and commensal bacteria. The first, found in the intracellular pathogen Chlamydia trachomatis, uses YhhQ and tRNA guanine transglycosylase (TGT) homologs that have changed substrate specificities to directly salvage q, mimicking the eukaryotic pathway. The second, found in bacteria from the gut flora such as Clostridioides difficile, salvages preQ1 from q through an unprecedented reaction catalyzed by a newly defined subgroup of the radical-SAM enzyme family. The source of q can be external through transport by members of the energy-coupling factor (ECF) family or internal through hydrolysis of Q by a dedicated nucleosidase. This work reinforces the concept that hosts and members of their associated microbiota compete for the salvage of Q precursors micronutrients.

Original languageEnglish (US)
Pages (from-to)19126-19135
Number of pages10
JournalProceedings of the National Academy of Sciences of the United States of America
Volume116
Issue number38
DOIs
StatePublished - Sep 17 2019

Fingerprint

Bacteria
queuine tRNA-ribosyltransferase
Enzymes
Eukaryota
Nucleoside Q
N-Glycosyl Hydrolases
Micronutrients
Chlamydia trachomatis
Microbiota
Recycling
Substrate Specificity
Transfer RNA
Hydrolysis
Escherichia coli
queuine
Proteins
Gastrointestinal Microbiome

Keywords

  • Comparative genomics
  • Nucleoside transport
  • Queuosine
  • RSAM
  • Sequence similarity network

ASJC Scopus subject areas

  • General

Cite this

Yuan, Y., Zallot, R., Grove, T. L., Payan, D. J., Martin-Verstraete, I., Šepić, S., ... de Crécy-Lagard, V. (2019). Discovery of novel bacterial queuine salvage enzymes and pathways in human pathogens. Proceedings of the National Academy of Sciences of the United States of America, 116(38), 19126-19135. https://doi.org/10.1073/pnas.1909604116

Discovery of novel bacterial queuine salvage enzymes and pathways in human pathogens. / Yuan, Yifeng; Zallot, Rémi; Grove, Tyler L.; Payan, Daniel J.; Martin-Verstraete, Isabelle; Šepić, Sara; Balamkundu, Seetharamsingh; Neelakandan, Ramesh; Gadi, Vinod K.; Liu, Chuan Fa; Swairjo, Manal A.; Dedon, Peter C.; Almo, Steven C.; Gerlt, John Alan; de Crécy-Lagard, Valérie.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, No. 38, 17.09.2019, p. 19126-19135.

Research output: Contribution to journalArticle

Yuan, Y, Zallot, R, Grove, TL, Payan, DJ, Martin-Verstraete, I, Šepić, S, Balamkundu, S, Neelakandan, R, Gadi, VK, Liu, CF, Swairjo, MA, Dedon, PC, Almo, SC, Gerlt, JA & de Crécy-Lagard, V 2019, 'Discovery of novel bacterial queuine salvage enzymes and pathways in human pathogens', Proceedings of the National Academy of Sciences of the United States of America, vol. 116, no. 38, pp. 19126-19135. https://doi.org/10.1073/pnas.1909604116
Yuan, Yifeng ; Zallot, Rémi ; Grove, Tyler L. ; Payan, Daniel J. ; Martin-Verstraete, Isabelle ; Šepić, Sara ; Balamkundu, Seetharamsingh ; Neelakandan, Ramesh ; Gadi, Vinod K. ; Liu, Chuan Fa ; Swairjo, Manal A. ; Dedon, Peter C. ; Almo, Steven C. ; Gerlt, John Alan ; de Crécy-Lagard, Valérie. / Discovery of novel bacterial queuine salvage enzymes and pathways in human pathogens. In: Proceedings of the National Academy of Sciences of the United States of America. 2019 ; Vol. 116, No. 38. pp. 19126-19135.
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AU - Šepić, Sara

AU - Balamkundu, Seetharamsingh

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AU - Liu, Chuan Fa

AU - Swairjo, Manal A.

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