Discovery of ERD-12310A as an Exceptionally Potent and Orally Efficacious PROTAC Degrader of Estrogen Receptor α (ERα)

Rohan Kalyan Rej, Biao Hu, Zhixiang Chen, Ranjan Kumar Acharyya, Dimin Wu, Hoda Metwally, Donna McEachern, Yu Wang, Wei Jiang, Longchuan Bai, Leticia S. Nishimura, Christina L. Gersch, Meilin Wang, Bo Wen, Duxin Sun, Kathryn Carlson, John A. Katzenellenbogen, Guozhang Xu, Weihong Zhang, Wenxue WuE. Scott Priestley, Zhihua Sui, James M. Rae, Shaomeng Wang

Research output: Contribution to journalArticlepeer-review

Abstract

Inhibition of estrogen receptor alpha (ERα) signaling is an established therapeutic approach for the treatment of ER-positive (ER+) breast cancers, but new therapeutic strategies are urgently needed to overcome clinical resistance. In the present study, we describe the discovery and extensive evaluation of ERD-12310A as an exceptionally potent and orally efficacious PROTAC degrader of ERα. ERD-12310A achieved a DC50 value of 47 pM and is 10 times more potent than ARV-471. ERD-12310A displayed an improved pharmacokinetic profile in mice and rats over ARV-471. ERD-12310A attained tumor regression in the ER+, estrogen-dependent MCF-7 breast cancer xenograft model with wild-type ER and is more potent than ARV-471. Importantly, ERD-12310A achieved strong tumor growth inhibition in MCF-7 xenograft tumors harboring the clinically relevant ESR1Y537S mutation, which confers resistance to traditional antiestrogens. Our data position ERD-12310A as a promising candidate for further development as a potential therapy for ER+ breast cancer.

Original languageEnglish (US)
Pages (from-to)20933-20965
Number of pages33
JournalJournal of Medicinal Chemistry
Volume67
Issue number23
Early online dateNov 25 2024
DOIs
StatePublished - Dec 12 2024

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery

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