Discovering new neuropeptides using single-cell mass spectrometry

Amanda B. Hummon, Jonathan V. Sweedler, Rebecca W. Corbin

Research output: Contribution to journalArticlepeer-review


Matrix-assisted laser desorption/ionization (MALDI) coupled with time-of-flight (TOF) mass spectrometry (MS) is a rapid and sensitive analytical approach well suited to obtaining molecular weights of peptides and proteins from single-cell samples. The current state of the art in single-cell neuropeptide profiling with MS is highlighted. Strategies for peptide identification and characterization of post-translational modifications are presented. Furthermore, several recent enhancements in MALDI-MS technology, including in situ peptide sequencing, as well as the direct spatial mapping of peptides in cells and tissues, are discussed.

Original languageEnglish (US)
Pages (from-to)515-521
Number of pages7
JournalTrAC - Trends in Analytical Chemistry
Issue number8
StatePublished - Sep 1 2003


  • Invertebrate
  • Mass spectrometry
  • Neuropeptide
  • Prohormone
  • Single-cell analysis

ASJC Scopus subject areas

  • Analytical Chemistry


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