Discovering new neuropeptides using single-cell mass spectrometry

Amanda B. Hummon; Jonathan V. Sweedler; Rebecca W. Corbin

doi:10.1016/S0165-9936(03)00901-4

Discovering new neuropeptides using single-cell mass spectrometry

Amanda B. Hummon, Jonathan V. Sweedler, Rebecca W. Corbin

Research output: Contribution to journal › Article › peer-review

Abstract

Matrix-assisted laser desorption/ionization (MALDI) coupled with time-of-flight (TOF) mass spectrometry (MS) is a rapid and sensitive analytical approach well suited to obtaining molecular weights of peptides and proteins from single-cell samples. The current state of the art in single-cell neuropeptide profiling with MS is highlighted. Strategies for peptide identification and characterization of post-translational modifications are presented. Furthermore, several recent enhancements in MALDI-MS technology, including in situ peptide sequencing, as well as the direct spatial mapping of peptides in cells and tissues, are discussed.

Original language	English (US)
Pages (from-to)	515-521
Number of pages	7
Journal	TrAC - Trends in Analytical Chemistry
Volume	22
Issue number	8
DOIs	https://doi.org/10.1016/S0165-9936(03)00901-4
State	Published - Sep 1 2003

Keywords

Invertebrate
MALDI
Mass spectrometry
Neuropeptide
Prohormone
Single-cell analysis

ASJC Scopus subject areas

Analytical Chemistry

Online availability

10.1016/S0165-9936(03)00901-4

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title = "Discovering new neuropeptides using single-cell mass spectrometry",

abstract = "Matrix-assisted laser desorption/ionization (MALDI) coupled with time-of-flight (TOF) mass spectrometry (MS) is a rapid and sensitive analytical approach well suited to obtaining molecular weights of peptides and proteins from single-cell samples. The current state of the art in single-cell neuropeptide profiling with MS is highlighted. Strategies for peptide identification and characterization of post-translational modifications are presented. Furthermore, several recent enhancements in MALDI-MS technology, including in situ peptide sequencing, as well as the direct spatial mapping of peptides in cells and tissues, are discussed.",

keywords = "Invertebrate, MALDI, Mass spectrometry, Neuropeptide, Prohormone, Single-cell analysis",

author = "Hummon, {Amanda B.} and Sweedler, {Jonathan V.} and Corbin, {Rebecca W.}",

note = "Funding Information: The authors gratefully acknowledge the National Institutes of Drug Abuse (DA14879) and the National Institute for Neurological Disease and Stroke (NS31609) for financial support. We thank Friday Harbor Laboratories for use of their facilities for the Tritonia peptide experiments. ",

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doi = "10.1016/S0165-9936(03)00901-4",

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AU - Hummon, Amanda B.

AU - Sweedler, Jonathan V.

AU - Corbin, Rebecca W.

N1 - Funding Information: The authors gratefully acknowledge the National Institutes of Drug Abuse (DA14879) and the National Institute for Neurological Disease and Stroke (NS31609) for financial support. We thank Friday Harbor Laboratories for use of their facilities for the Tritonia peptide experiments.

PY - 2003/9/1

Y1 - 2003/9/1

N2 - Matrix-assisted laser desorption/ionization (MALDI) coupled with time-of-flight (TOF) mass spectrometry (MS) is a rapid and sensitive analytical approach well suited to obtaining molecular weights of peptides and proteins from single-cell samples. The current state of the art in single-cell neuropeptide profiling with MS is highlighted. Strategies for peptide identification and characterization of post-translational modifications are presented. Furthermore, several recent enhancements in MALDI-MS technology, including in situ peptide sequencing, as well as the direct spatial mapping of peptides in cells and tissues, are discussed.

AB - Matrix-assisted laser desorption/ionization (MALDI) coupled with time-of-flight (TOF) mass spectrometry (MS) is a rapid and sensitive analytical approach well suited to obtaining molecular weights of peptides and proteins from single-cell samples. The current state of the art in single-cell neuropeptide profiling with MS is highlighted. Strategies for peptide identification and characterization of post-translational modifications are presented. Furthermore, several recent enhancements in MALDI-MS technology, including in situ peptide sequencing, as well as the direct spatial mapping of peptides in cells and tissues, are discussed.

KW - Invertebrate

KW - MALDI

KW - Mass spectrometry

KW - Neuropeptide

KW - Prohormone

KW - Single-cell analysis

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JO - TrAC - Trends in Analytical Chemistry

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Discovering new neuropeptides using single-cell mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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