Disassembly of transcriptional regulatory complexes by molecular chaperones

Brian C. Freeman, Keith R. Yamamoto

Research output: Contribution to journalArticle

Abstract

Many biological processes are initiated by cooperative assembly of large multi-component complexes; however, mechanisms for modulating or terminating the actions of these complexes are not well understood. For example, hormone-bound intracellular receptors (IRs) nucleate formation of transcriptional regulatory complexes whose actions cease promptly upon hormone withdrawal. Here, we show that the p23 molecular chaperone localizes in vivo to genomic response elements in a hormone-dependent manner, disrupting receptor-mediated transcriptional activation in vivo and in vitro; Hsp90 weakly displayed similar activities. Indeed, p23 and Hsp90 also disrupted the activities of some non-IR-containing transcriptional regulatory complexes. We suggest that molecular chaperones promote disassembly of transcriptional regulatory complexes, thus enabling regulatory machineries to detect and respond to signaling changes.

Original languageEnglish (US)
Pages (from-to)2232-2235
Number of pages4
JournalScience
Volume296
Issue number5576
DOIs
StatePublished - Jun 21 2002
Externally publishedYes

ASJC Scopus subject areas

  • General

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