Disassembly of nanodiscs with cholate

Amy Y. Shih, Peter L. Freddolino, Stephen G. Sligar, Klaus Schulten

Research output: Contribution to journalArticle


Nanodiscs are protein-lipid particles that furnish a nanometer-sized membrane environment for the investigation of membrane proteins. Nanodiscs assemble spontaneously upon the removal of cholate from an initial mixture of cholate, lipids, and engineered amphipathic proteins. A combined experimental-computational approach is applied here to study the disassembly of nanodiscs through the addition of cholate to preformed particles. For this purpose, small-angle X-ray scattering experiments and coarse-grained molecular dynamics simulations were performed and compared. The study offers a detailed view of nanodisc dynamics.

Original languageEnglish (US)
Pages (from-to)1692-1696
Number of pages5
JournalNano letters
Issue number6
StatePublished - Jun 1 2007

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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    Shih, A. Y., Freddolino, P. L., Sligar, S. G., & Schulten, K. (2007). Disassembly of nanodiscs with cholate. Nano letters, 7(6), 1692-1696. https://doi.org/10.1021/nl0706906