Disassembly of nanodiscs with cholate

Amy Y. Shih, Peter L. Freddolino, Stephen G. Sligar, Klaus Schulten

Research output: Contribution to journalArticlepeer-review

Abstract

Nanodiscs are protein-lipid particles that furnish a nanometer-sized membrane environment for the investigation of membrane proteins. Nanodiscs assemble spontaneously upon the removal of cholate from an initial mixture of cholate, lipids, and engineered amphipathic proteins. A combined experimental-computational approach is applied here to study the disassembly of nanodiscs through the addition of cholate to preformed particles. For this purpose, small-angle X-ray scattering experiments and coarse-grained molecular dynamics simulations were performed and compared. The study offers a detailed view of nanodisc dynamics.

Original languageEnglish (US)
Pages (from-to)1692-1696
Number of pages5
JournalNano letters
Volume7
Issue number6
DOIs
StatePublished - Jun 2007

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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