Abstract
We report here the first use of directed mutagenesis in Methanosarcina acetivorans C2A. The method employs homologous recombination-mediated gene replacement and was used to construct a variety of proline auxotrophs with mutations in the proABC locus. Each mutation was also complemented in trans with autonomously replicating Methanosarcina-Escherichia plasmid shuttle vectors.
Original language | English (US) |
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Pages (from-to) | 1449-1454 |
Number of pages | 6 |
Journal | Journal of bacteriology |
Volume | 184 |
Issue number | 5 |
DOIs | |
State | Published - 2002 |
ASJC Scopus subject areas
- Microbiology
- Molecular Biology