We report here the first use of directed mutagenesis in Methanosarcina acetivorans C2A. The method employs homologous recombination-mediated gene replacement and was used to construct a variety of proline auxotrophs with mutations in the proABC locus. Each mutation was also complemented in trans with autonomously replicating Methanosarcina-Escherichia plasmid shuttle vectors.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of bacteriology|
|State||Published - 2002|
ASJC Scopus subject areas
- Molecular Biology