Abstract
Directed evolution has been successfully used to engineer proteins for basic and applied biological research. However, engineering of novel protein functions by directed evolution remains an overwhelming challenge. This challenge may come from the fact that multiple simultaneously or synergistic mutations are required for the creation of a novel protein function. Here we review the key developments in engineering of novel protein functions by using either directed evolution or a combined directed evolution and rational or computational design approach. Specific attention will be paid to a molecular evolution model for generation of novel proteins. The engineered novel proteins should not only broaden the range of applications of proteins but also provide new insights into protein structure-function relationship and protein evolution.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 313-317 |
| Number of pages | 5 |
| Journal | Biotechnology and bioengineering |
| Volume | 98 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 1 2007 |
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Keywords
- Directed evolution
- Promiscuous functions
- Proteins
- Rational design
ASJC Scopus subject areas
- Biotechnology
- Microbiology
Cite this
Directed evolution of novel protein functions. / Zhao, Huimin.
In: Biotechnology and bioengineering, Vol. 98, No. 2, 01.10.2007, p. 313-317.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Directed evolution of novel protein functions
AU - Zhao, Huimin
PY - 2007/10/1
Y1 - 2007/10/1
N2 - Directed evolution has been successfully used to engineer proteins for basic and applied biological research. However, engineering of novel protein functions by directed evolution remains an overwhelming challenge. This challenge may come from the fact that multiple simultaneously or synergistic mutations are required for the creation of a novel protein function. Here we review the key developments in engineering of novel protein functions by using either directed evolution or a combined directed evolution and rational or computational design approach. Specific attention will be paid to a molecular evolution model for generation of novel proteins. The engineered novel proteins should not only broaden the range of applications of proteins but also provide new insights into protein structure-function relationship and protein evolution.
AB - Directed evolution has been successfully used to engineer proteins for basic and applied biological research. However, engineering of novel protein functions by directed evolution remains an overwhelming challenge. This challenge may come from the fact that multiple simultaneously or synergistic mutations are required for the creation of a novel protein function. Here we review the key developments in engineering of novel protein functions by using either directed evolution or a combined directed evolution and rational or computational design approach. Specific attention will be paid to a molecular evolution model for generation of novel proteins. The engineered novel proteins should not only broaden the range of applications of proteins but also provide new insights into protein structure-function relationship and protein evolution.
KW - Directed evolution
KW - Promiscuous functions
KW - Proteins
KW - Rational design
UR - http://www.scopus.com/inward/record.url?scp=34948853912&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34948853912&partnerID=8YFLogxK
U2 - 10.1002/bit.21455
DO - 10.1002/bit.21455
M3 - Review article
C2 - 17721862
AN - SCOPUS:34948853912
VL - 98
SP - 313
EP - 317
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 2
ER -