Directed evolution of homing endonuclease with novel DNA sequence specificity

Keyphrases

• Directed Evolution 100%
• Homing Endonuclease 100%
• DNA Sequence Specificity 100%
• I-SceI 57%
• Selection Strategy 42%
• Sequence Specificity 28%
• Targeted Sequencing 28%
• Cystic Fibrosis 28%
• Gene Therapy 28%
• DNA Sequencing 14%
• Highly Sensitive 14%
• Gene-gene 14%
• Conductance 14%
• Transmembrane 14%
• Escherichia Coli 14%
• Metabolic Engineering 14%
• Systems Biology 14%
• Survival Rate 14%
• Homologous Recombination 14%
• DNA Cleavage 14%
• Double-strand Breaks 14%
• Mutated Genes 14%
• OR Genes 14%
• Specific Conditions 14%
• Recognition Site 14%
• Cell Survival Rate 14%
• Novel Sequences 14%
• Gene Alteration 14%
• CFTR Gene 14%
• Gene Correction 14%
• Efficient Selection 14%

Biochemistry, Genetics and Molecular Biology

• DNA Sequence 100%
• Homing Endonuclease 100%
• Enzyme 28%
• Survival Rate 28%
• Cystic Fibrosis 28%
• Gene Therapy 28%
• Metabolic Engineering 14%
• Escherichia coli 14%
• Coevolution 14%
• Wild Type 14%
• DNA Cleavage 14%
• Homologous Recombination 14%
• Cell Survival 14%
• Gene Mutation 14%
• Cystic Fibrosis Transmembrane Conductance Regulator 14%