TY - JOUR
T1 - Directed evolution of enzymes and biosynthetic pathways
AU - Johannes, Tyler W.
AU - Zhao, Huimin
N1 - Funding Information:
We gratefully acknowledge financial support from the Biotechnology Research and Development Consortium (BRDC) (Project 2-4-121), Office of Naval Research (N00014-02-1-0725), National Science Foundation (BES-0348107) and Department of Energy. TJ also acknowledges fellowship support from the DuPont Company.
PY - 2006/6
Y1 - 2006/6
N2 - Directed evolution is an important tool for overcoming the limitations of natural enzymes as biocatalysts. Recent advances have focused on applying directed evolution to a variety of enzymes, such as epoxide hydrolase, glyphosate N-acetyltransferase, xylanase and phosphotriesterase, in order to improve their activity, selectivity, stability and solubility. The focus has also shifted to manipulating biosynthetic pathways for the production of many naturally synthesized compounds, as well as the production of novel 'unnatural' compounds. A combined directed evolution and computational design approach is becoming increasingly important in exploring enzyme sequence-space and creating improved or novel enzymes. Fueled by recent breakthroughs in genomics and metagenomics, these developments should help expand the use of biocatalysts in industry.
AB - Directed evolution is an important tool for overcoming the limitations of natural enzymes as biocatalysts. Recent advances have focused on applying directed evolution to a variety of enzymes, such as epoxide hydrolase, glyphosate N-acetyltransferase, xylanase and phosphotriesterase, in order to improve their activity, selectivity, stability and solubility. The focus has also shifted to manipulating biosynthetic pathways for the production of many naturally synthesized compounds, as well as the production of novel 'unnatural' compounds. A combined directed evolution and computational design approach is becoming increasingly important in exploring enzyme sequence-space and creating improved or novel enzymes. Fueled by recent breakthroughs in genomics and metagenomics, these developments should help expand the use of biocatalysts in industry.
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U2 - 10.1016/j.mib.2006.03.003
DO - 10.1016/j.mib.2006.03.003
M3 - Review article
C2 - 16621678
AN - SCOPUS:33744512606
SN - 1369-5274
VL - 9
SP - 261
EP - 267
JO - Current Opinion in Microbiology
JF - Current Opinion in Microbiology
IS - 3
ER -