Directed Evolution of CRISPR-Cas9 Base Editors

Jackson Winter, Pablo Perez-Pinera

Research output: Contribution to journalShort survey

Abstract

A recent publication by Thuronyi et al. described a directed evolution system called phage-assisted continuous evolution (PACE) that was used to generate improved variants of CRISPR-Cas9 base editors. These evolved base editors overcome some of the inherent limitations of the technology such as sequence context preferences, restricted editing windows, and large construct sizes.

Original languageEnglish (US)
Pages (from-to)1151-1153
Number of pages3
JournalTrends in Biotechnology
Volume37
Issue number11
DOIs
StatePublished - Nov 2019

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Clustered Regularly Interspaced Short Palindromic Repeats
Bacteriophages
Technology

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Directed Evolution of CRISPR-Cas9 Base Editors. / Winter, Jackson; Perez-Pinera, Pablo.

In: Trends in Biotechnology, Vol. 37, No. 11, 11.2019, p. 1151-1153.

Research output: Contribution to journalShort survey

Winter, Jackson ; Perez-Pinera, Pablo. / Directed Evolution of CRISPR-Cas9 Base Editors. In: Trends in Biotechnology. 2019 ; Vol. 37, No. 11. pp. 1151-1153.
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