Directed Evolution of CRISPR-Cas9 Base Editors

Jackson Winter; Pablo Perez-Pinera

doi:10.1016/j.tibtech.2019.09.005

Directed Evolution of CRISPR-Cas9 Base Editors

Research output: Contribution to journal › Short survey › peer-review

Abstract

A recent publication by Thuronyi et al. described a directed evolution system called phage-assisted continuous evolution (PACE) that was used to generate improved variants of CRISPR-Cas9 base editors. These evolved base editors overcome some of the inherent limitations of the technology such as sequence context preferences, restricted editing windows, and large construct sizes.

Original language	English (US)
Pages (from-to)	1151-1153
Number of pages	3
Journal	Trends in Biotechnology
Volume	37
Issue number	11
DOIs	https://doi.org/10.1016/j.tibtech.2019.09.005
State	Published - Nov 2019

ASJC Scopus subject areas

Biotechnology
Bioengineering

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10.1016/j.tibtech.2019.09.005

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title = "Directed Evolution of CRISPR-Cas9 Base Editors",

abstract = "A recent publication by Thuronyi et al. described a directed evolution system called phage-assisted continuous evolution (PACE) that was used to generate improved variants of CRISPR-Cas9 base editors. These evolved base editors overcome some of the inherent limitations of the technology such as sequence context preferences, restricted editing windows, and large construct sizes.",

author = "Jackson Winter and Pablo Perez-Pinera",

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year = "2019",

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AB - A recent publication by Thuronyi et al. described a directed evolution system called phage-assisted continuous evolution (PACE) that was used to generate improved variants of CRISPR-Cas9 base editors. These evolved base editors overcome some of the inherent limitations of the technology such as sequence context preferences, restricted editing windows, and large construct sizes.

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Directed Evolution of CRISPR-Cas9 Base Editors

Abstract

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