TY - JOUR
T1 - Directed Evolution
T2 - Methodologies and Applications
AU - Wang, Yajie
AU - Xue, Pu
AU - Cao, Mingfeng
AU - Yu, Tianhao
AU - Lane, Stephan T.
AU - Zhao, Huimin
N1 - Funding Information:
We acknowledge financial support from the U.S. Department of Energy (DE-SC0018420 and DE-SC0018260) and the National Science Foundation (Award No. 2019897) for various directed evolution projects (H.Z.). The online tool of BioRender ( BioRender.com ) was used to create parts of Figures 6, 8–16, and 22.
Publisher Copyright:
© 2021 American Chemical Society.
PY - 2021/10/27
Y1 - 2021/10/27
N2 - Directed evolution aims to expedite the natural evolution process of biological molecules and systems in a test tube through iterative rounds of gene diversifications and library screening/selection. It has become one of the most powerful and widespread tools for engineering improved or novel functions in proteins, metabolic pathways, and even whole genomes. This review describes the commonly used gene diversification strategies, screening/selection methods, and recently developed continuous evolution strategies for directed evolution. Moreover, we highlight some representative applications of directed evolution in engineering nucleic acids, proteins, pathways, genetic circuits, viruses, and whole cells. Finally, we discuss the challenges and future perspectives in directed evolution.
AB - Directed evolution aims to expedite the natural evolution process of biological molecules and systems in a test tube through iterative rounds of gene diversifications and library screening/selection. It has become one of the most powerful and widespread tools for engineering improved or novel functions in proteins, metabolic pathways, and even whole genomes. This review describes the commonly used gene diversification strategies, screening/selection methods, and recently developed continuous evolution strategies for directed evolution. Moreover, we highlight some representative applications of directed evolution in engineering nucleic acids, proteins, pathways, genetic circuits, viruses, and whole cells. Finally, we discuss the challenges and future perspectives in directed evolution.
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U2 - 10.1021/acs.chemrev.1c00260
DO - 10.1021/acs.chemrev.1c00260
M3 - Review article
C2 - 34297541
AN - SCOPUS:85112375378
SN - 0009-2665
VL - 121
SP - 12384
EP - 12444
JO - Chemical Reviews
JF - Chemical Reviews
IS - 20
ER -