TY - JOUR
T1 - Directed evolution
T2 - An evolving and enabling synthetic biology tool
AU - Cobb, Ryan E.
AU - Si, Tong
AU - Zhao, Huimin
N1 - Funding Information:
We gratefully acknowledge financial support from the BP Energy Biosciences Institute , National Institutes of Health ( GM077596 ), and National Academies Keck Futures Initiative on Synthetic Biology .
PY - 2012/8
Y1 - 2012/8
N2 - Synthetic biology, with its goal of designing biological entities for wide-ranging purposes, remains a field of intensive research interest. However, the vast complexity of biological systems has heretofore rendered rational design prohibitively difficult. As a result, directed evolution remains a valuable tool for synthetic biology, enabling the identification of desired functionalities from large libraries of variants. This review highlights the most recent advances in the use of directed evolution in synthetic biology, focusing on new techniques and applications at the pathway and genome scale.
AB - Synthetic biology, with its goal of designing biological entities for wide-ranging purposes, remains a field of intensive research interest. However, the vast complexity of biological systems has heretofore rendered rational design prohibitively difficult. As a result, directed evolution remains a valuable tool for synthetic biology, enabling the identification of desired functionalities from large libraries of variants. This review highlights the most recent advances in the use of directed evolution in synthetic biology, focusing on new techniques and applications at the pathway and genome scale.
UR - http://www.scopus.com/inward/record.url?scp=84865279828&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865279828&partnerID=8YFLogxK
U2 - 10.1016/j.cbpa.2012.05.186
DO - 10.1016/j.cbpa.2012.05.186
M3 - Review article
C2 - 22673064
AN - SCOPUS:84865279828
SN - 1367-5931
VL - 16
SP - 285
EP - 291
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
IS - 3-4
ER -